HMGB1/HMG-1 Antibody (19N10B7) - BSA Free

Novus Biologicals | Catalog # NBP2-27396

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (99%), Canine (100%), Chicken (91%), Chinese Hamster (99%), Equine (100%), Porcine (99%), Primate (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Simple Western, Immunoprecipitation, CyTOF-ready

Cited:

Immunoprecipitation, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 19N10B7

Format

BSA Free
View all HMGB1/HMG-1 Primary Antibodies »

Product Specifications

Immunogen

Full-length recombinant human HMGB1 protein

Reactivity Notes

Based upon immunogen sequence similarity, this antibody is predicted to react with Rat (99%), Bovine (99%), Porcine/Pig (99%), Chinese Hamster (99%), Chicken (91%), Canine/Dog (100%), Primate/Monkey (100%) and Equine/Horse (100%). Mouse reactivity reported in a verified customer review.

Localization

Nucleus (very high), Cytoplasm (very low)

Specificity

This antibody recognizes both A box domain (amino acids 9-79) and full length HMGB1 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for HMGB1/HMG-1 Antibody (19N10B7) - BSA Free

Western Blot: HMGB1/HMG-1 Antibody (19N10B7)BSA Free [NBP2-27396]

Western Blot: HMGB1/HMG-1 Antibody (19N10B7)BSA Free [NBP2-27396]

Western Blot: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Hepatocyte protein lysate at 1:1000 4C overnight. Image from verified customer review.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed, paraffin-embedded human liver tissue stained with HMGB1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Flow Cytometry: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Flow Cytometry: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Flow Cytometry: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Intracellular analysis using HMGB1 antibody. Human Jurkat cells were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green).
Western Blot: HMGB1/HMG-1 Antibody (19N10B7)BSA Free [NBP2-27396]

Western Blot: HMGB1/HMG-1 Antibody (19N10B7)BSA Free [NBP2-27396]

Western Blot: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Analysis using 2 ug/ml concentration of HMGB1 antibody (clone 19N10B7) on (A) Full-length human HMGB1 protein, (B) Human Jurkat cell lysate and (C) Mouse NIH 3T3 cell lysate. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution was used for this test.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed, paraffin-embedded human spleen tissue stained with HMGB1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed, paraffin-embedded human breast stained with HMGB1 antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Formalin-fixed paraffin-embedded human prostate tissue stained with HMGB1 antibody at 10 ug/ml concentration. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Analysis of formalin-fixed, decalcified, paraffin embedded tissue section from the paws of mouse (collagen-induced arthritis model) using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. [Image courtesy of Dr Ulf Andersson, Karolinska Institute].
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of human stomach using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Distinct nuclear staining of HMGB1 was observed in different cells of the glandular stomach.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of normal human skin using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. The various cells of the epidermal layer showed intense nuclear staining along with weak cytoplasmic staining. The blood vessels, glandular cells and the other cells in dermal layer also showed nuclear positivity for HMGB1 immunostaining.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of normal human brain using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Representative image shows a distinct nuclear immunostaining of HMGB1 in the various brain cells.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of normal human colon using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Representative image shows specific HMGB1 nuclear positivity in different mucosal cells of the colon.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of malignant stromal tumor of small bowel from human using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. HMGB1 immunopositivity of differential intensity was observed in the cells of tested section.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of human bladder cancer using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. Strong nuclear HMGB1 immunopositivity was observed in the bladder cancer cells whereas the staining was weak in cells of tumor stroma.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) - BSA Free [NBP2-27396]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - IHC-P detection of HMGB1 protein in a formalin-fixed paraffin-embedded tissue section of human stomach cancer using HMGB1 antibody (clone 19N10B7) at 5 ug/ml concentration. This representative image shows a distinct nuclear HMGB1 immunopositivity in the cancerous and sub-mucosal cells.
Simple Western: HMGB1/HMG-1 Antibody (19N10B7)BSA Free [NBP2-27396]

Simple Western: HMGB1/HMG-1 Antibody (19N10B7)BSA Free [NBP2-27396]

Simple Western: HMGB1/HMG-1 Antibody (19N10B7) [NBP2-27396] - Simple Western lane view shows a specific band for HMGB1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Applications for HMGB1/HMG-1 Antibody (19N10B7) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5ug / 5x10^5cells

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

reported in scientific literature (PMID 23696858)

Simple Western

1:50

Western Blot

1-3 ug/ml. Use reported by customer review
Application Notes
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 34 kDa. This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 5 using NBP2-27396 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody (19N10B7) - BSA Free

Certificate of Analysis

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Product Specific Notices for HMGB1/HMG-1 Antibody (19N10B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HMGB1/HMG-1 Antibody (19N10B7) - BSA Free

Customer Reviews for HMGB1/HMG-1 Antibody (19N10B7) - BSA Free (1)

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  • Name: Liyong Zhang
    Application: Western Blot
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 06/26/2014
    A review for HMGB1 antibody (HMGB1-NBP1-40650)
    HMGB1/HMG-1 Antibody (19N10B7) - BSA Free NBP2-27396

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