HMGB1/HMG-1 Antibody (19N15F4) - BSA Free

Novus Biologicals | Catalog # NBP2-27401

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Canine (100%), Chicken (91%), Chinese Hamster (99%), Equine (99%), Porcine (99%), Primate (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 19N15F4

Format

BSA Free
View all HMGB1/HMG-1 Primary Antibodies »

Product Specifications

Immunogen

Full-length recombinant human HMGB1 was used as the immunogen for the antibody.

Reactivity Notes

Based upon immunogen sequence similarity, this antibody is predicted to react 100% with olive baboon, crab-eating macaque, and common marmoset.

Localization

Nucleus (very high, ubiquitous), Cytoplasmic (very low, some tissues)

Specificity

This antibody recognizes both the box A domain (amino acids 9-79) and full length HMGB1 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for HMGB1/HMG-1 Antibody (19N15F4) - BSA Free

Western Blot: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Western Blot: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Western Blot: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein on ( A) Full-length human HMGB1 protein (B) human Jurkat cell lysate and (C) mouse NIH 3T3 cell lysate using HMGB1 antibody (clone 19N15F4) at 2 ug/ml concentration. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used in this assay.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - IHC-P detection of HMGB1 protein in formalin-fixed, decalcified, paraffin embedded tissue sections from the paws of mouse (collagen-induced arthritis model) using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml [Image courtesy of Dr Ulf Andersson, Karolinska]
Flow Cytometry: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Flow Cytometry: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Flow Cytometry: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Intracellular analysis using HMGB1 antibody. Human Jurkat cells were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green).
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Formalin-fixed, paraffin-embedded human skin stained with HMGB1 antibody at 5 ug/ml
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a section of human breast normal tissue using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. The ductal /acinar epithelial cells in the breast tissue section depicted strong nuclear expression with some cytoplasmic positivity. The myoepithelial cells and few cells of the intra-lobular connective tissue showed relatively weak nuclear positivity for HMGB1.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a section of normal human stomach tissue using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. The cells of the glandular stomach showed specific nuclear expression for HMGB1.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a tissue section of normal human colon using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. Almost all the cells of colon's mucosal layer showed nuclear positivity for HMGB1 but the cells of the columnar epithelial cells on the absorptive surface developed cytoplasmic staining also.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a tissue section of normal human brain using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. The representative image shows an overall strong nuclear HMGB1 immunopositivity with weak to negligible cytoplasmic staining in brain cells.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a tissue section of human bladder transitional cell carcinoma (TCC) / urothelial cell carcinoma (UCC) using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. Almost all the cancer cells and the cells of tumor stroma developed strong/specific nuclear HMGB1 immunopositivity.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a tissue section of human esophageal squamous cell carcinoma (SCC) using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. Strong nuclear along with weak cytoplasmic immunopositivity for HMGB1 was observed in SCC cells and the tumor stroma cells developed relatively weak staining for this target.
Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401]

Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (19N15F4) [NBP2-27401] - Analysis of HMGB1 protein in a section of human small intestinal cancer tissue using HMGB1 antibody (clone 19N15F4) at a concentration of 5 ug/ml. Intense nuclear immunopositivity of HMGB1 was observed in cancer cells and the cells of tumor stroma as well as the adjacent normal crypts. Some glandular cells in the crypts developed bother nuclear and cytoplasmic staining.

Applications for HMGB1/HMG-1 Antibody (19N15F4) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5ug/5x10^5cells~

Immunohistochemistry-Paraffin

5ug/ml

Western Blot

1-3ug/ml~

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB1/HMG-1

HMGB1 (high mobility group protein B1) belongs to the HMGB family and contains two HMG box DNA-binding domains. It is a highly conserved, ubiquitous protein present in the nuclei and cytoplasm of nearly all cell types, and is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). It is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Gene Symbol

HMGB1

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody (19N15F4) - BSA Free

Certificate of Analysis

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Product Specific Notices for HMGB1/HMG-1 Antibody (19N15F4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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