HNF-3 beta/FoxA2 Antibody (6C12) - Azide and BSA Free

Novus Biologicals | Catalog # H00003170-M12

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6C12

Format

Azide and BSA Free
View all HNF-3 beta/FoxA2 Primary Antibodies »

Product Specifications

Immunogen

FOXA2 (NP_068556, 363 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HLKPEHHYAFNHPFSINNLMSSEQQHHHSHHHHQPHKMDLKAYEQVMHYPGYGSPMPGSLAMGPVTNKTGLDASPLAADTSYYQGVYSRPIMNSS

Specificity

forkhead box A2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HNF-3 beta/FoxA2 Antibody (6C12) - Azide and BSA Free

Western Blot: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12]

Western Blot: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12]

Western Blot: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12] - Analysis of FOXA2 expression in K-562 (Cat # L009V1).
Immunocytochemistry/ Immunofluorescence: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12]

Immunocytochemistry/ Immunofluorescence: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12]

Immunocytochemistry/Immunofluorescence: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12] - Analysis of monoclonal antibody to FOXA2 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12]

Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12]

Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12] - Analysis of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 0.5 ug/ml
ELISA: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12]

ELISA: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12]

ELISA: HNF-3 beta/FoxA2 Antibody (6C12) [H00003170-M12] - Detection limit for recombinant GST tagged FOXA2 is approximately 0.3ng/ml as a capture antibody.

Applications for HNF-3 beta/FoxA2 Antibody (6C12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID:34280890). Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HNF-3 beta/FoxA2

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Long Name

Hepatocyte Nuclear Factor-3, beta

Alternate Names

FoxA2, HNF3 beta

Entrez Gene IDs

3170 (Human)

Gene Symbol

FOXA2

OMIM

600288 (Human)

UniProt

NP_068556 (Human)

Additional HNF-3 beta/FoxA2 Products

Product Documents for HNF-3 beta/FoxA2 Antibody (6C12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HNF-3 beta/FoxA2 Antibody (6C12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HNF-3 beta/FoxA2 Antibody (6C12) - Azide and BSA Free

Customer Reviews for HNF-3 beta/FoxA2 Antibody (6C12) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review HNF-3 beta/FoxA2 Antibody (6C12) - Azide and BSA Free and earn rewards!

Have you used HNF-3 beta/FoxA2 Antibody (6C12) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HNF-3 beta/FoxA2 Antibody (6C12) - Azide and BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I want to use the FoxA2 antibody (H00003170-M12) for Immunohistochemistry-Paraffin. Therefore I'm wondering if you can tell me which protocol you used to generate your picture? Which dilution did you take and which antigen retrieval method did you use in this case?

    A:

    Here is the link to the protocol that Abnova (the company that produces this antibody) uses on their site listed under the product (http://www.abnova.com/protocol_pdf/Immunohistochemistry.pdf). The dilution is 0.5 ug/mL. I would recommend citrate buffer pH 6.0 as the antigen retrieval as that is the one our lab has a lot of success with. We use a pressure cooker for the citrate buffer antigen retrieval method.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies