hnRNP A1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32161

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all hnRNP A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human hnRNP A1. The exact sequence is proprietary.

Localization

Nucleus, Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for hnRNP A1 Antibody - BSA Free

Western Blot: hnRNP A1 Antibody [NBP1-32161]

Western Blot: hnRNP A1 Antibody [NBP1-32161]

Western Blot: hnRNP A1 Antibody [NBP1-32161] - Wild-type (WT) and hnRNP A1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A1 antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: hnRNP A1 Antibody [NBP1-32161]

Immunocytochemistry/ Immunofluorescence: hnRNP A1 Antibody [NBP1-32161]

Immunocytochemistry/Immunofluorescence: hnRNP A1 Antibody [NBP1-32161] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: hnRNP A1 Antibody [NBP1-32161]

Immunohistochemistry-Paraffin: hnRNP A1 Antibody [NBP1-32161]

Immunohistochemistry-Paraffin: hnRNP A1 Antibody [NBP1-32161] - hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. hnRNP A1 antibody diluted at 1:500.
Western Blot: hnRNP A1 Antibody [NBP1-32161]

Western Blot: hnRNP A1 Antibody [NBP1-32161]

Western Blot: hnRNP A1 Antibody [NBP1-32161] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: hnRNP A1 Antibody [NBP1-32161]

Western Blot: hnRNP A1 Antibody [NBP1-32161]

Western Blot: hnRNP A1 Antibody [NBP1-32161] - Sample(30 ug of whole cell lysate) A:H1299 B:HeLa S3 12% SDS PAGE, antibody diluted at 1:500.
Western Blot: hnRNP A1 Antibody [NBP1-32161]

Western Blot: hnRNP A1 Antibody [NBP1-32161]

Western Blot: hnRNP A1 Antibody [NBP1-32161] - A. 30 ug PC-12 whole cell lysate/extract.
hnRNP A1 Antibody

Immunohistochemistry: hnRNP A1 Antibody [NBP1-32161] -

Immunohistochemistry: hnRNP A1 Antibody [NBP1-32161] - hnRNP A1 antibody detects hnRNP A1 protein at nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
hnRNP A1 antibody (NBP1-32161) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Applications for hnRNP A1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: hnRNP A1

This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites. [provided by RefSeq]

Alternate Names

Helix-destabilizing protein, heterogeneous nuclear ribonucleoprotein A1, heterogeneous nuclear ribonucleoprotein A1B protein, heterogeneous nuclear ribonucleoprotein B2 protein, heterogeneous nuclear ribonucleoprotein core protein A1, hnRNP A1, hnRNP core protein A1, hnRNPA1, hnRNP-A1, HNRPA1MGC102835, nuclear ribonucleoprotein particle A1 protein, single-strand DNA-binding protein UP1, Single-strand RNA-binding protein

Gene Symbol

HNRNPA1

Additional hnRNP A1 Products

Product Documents for hnRNP A1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for hnRNP A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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