HO-1/HMOX1/HSP32 Antibody (SP08-07)
Novus Biologicals | Catalog # NBP2-67382
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382]](https://resources.rndsystems.com/images/products/HO-1-HMOX1-HSP32-Antibody-SP08-07-Immunohistochemistry-Paraffin-NBP2-67382-img0004.jpg "Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SP08-07 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within Human HO-1/HMOX1/HSP32 aa 227-276 / 288. (SwissProt: P09601 Human)
Localization
Microsome, Endoplasmic reticulum membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HO-1/HMOX1/HSP32 Antibody (SP08-07)
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382]
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded mouse liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.![Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382]](https://resources.rndsystems.com/images/products/HO-1-HMOX1-HSP32-Antibody-SP08-07-Flow-Cytometry-NBP2-67382-img0001.jpg "Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382]")
Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382]
Flow Cytometry: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of Jurkat cells with HO-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382]](https://resources.rndsystems.com/images/products/HO-1-HMOX1-HSP32-Antibody-SP08-07-Immunohistochemistry-Paraffin-NBP2-67382-img0002.jpg "Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382]")
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382]
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded human liver tissue using anti-HO-1 antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382]](https://resources.rndsystems.com/images/products/HO-1-HMOX1-HSP32-Antibody-SP08-07-Immunohistochemistry-Paraffin-NBP2-67382-img0003.jpg "Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382]")
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382]
Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (SP08-07) [NBP2-67382] - Analysis of paraffin-embedded human spleen tissue using anti-HO-1 antibody. Counter stained with hematoxylin.Applications for HO-1/HMOX1/HSP32 Antibody (SP08-07)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:1000
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HO-1/HMOX1/HSP32
Long Name
Heme Oxygenase 1
Alternate Names
HMOX1, HO1, HSP32
Gene Symbol
HMOX1
Additional HO-1/HMOX1/HSP32 Products
Product Documents for HO-1/HMOX1/HSP32 Antibody (SP08-07)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HO-1/HMOX1/HSP32 Antibody (SP08-07)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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