H.O.H.(TM) (Human on Human) Immunodetection Kit
Novus Biologicals | Catalog # HOH-3000-NB
Key Product Details
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Applications
Kit Type
Product Summary for H.O.H.(TM) (Human on Human) Immunodetection Kit
Features:
The H.O.H. Immunodetection Kit contains enough stock reagents to produce about 5 mL of working solution, which is generally sufficient to stain approximately 50 - 100 tissue sections.
Product Specifications
Application Notes
Scientific Data Images for H.O.H.(TM) (Human on Human) Immunodetection Kit
Immunohistochemistry-Paraffin: H.O.H.(TM) (Human on Human) Immunodetection Kit [HOH-3000-NB]
Immunohistochemistry-Paraffin: H.O.H.(TM) (Human on Human) Immunodetection Kit [HOH-3000-NB] - Serial sections of human kidney (FFPE). Left image shows strong, specific staining(brown) using human anti-cytokeratin primary antibody and detected withHOH-3000. Right image is a negative control showing absence of staining (nobackground) with the omission of just the primary antibody in the HOH-3000assay. Both sections counterstained with hematoxylin (blue nuclei).Kit Contents for H.O.H.(TM) (Human on Human) Immunodetection Kit
- HRP anti-Goat IgG - 300 uL
- HRP Antibody Diluent - 5 mL
- ImmPACT DAB EqV Reagent 1 (Chromogen) - 2.5 mL
- ImmPACT DAB EqV Reagent 2 (Diluent) - 2.5 mL
- Protein Block - 5 mL
- Solution A - 2.5 mL
- Solution B - 2.5 mL
Formulation, Preparation, and Storage
Concentration
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Product Documents for H.O.H.(TM) (Human on Human) Immunodetection Kit
Certificate of Analysis
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Product Specific Notices for H.O.H.(TM) (Human on Human) Immunodetection Kit
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars