HOIP/RNF31 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-55059

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HOIP/RNF31 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to RNF31(ring finger protein 31) The peptide sequence was selected from the middle region of RNF31. Peptide sequence SLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKL. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for HOIP/RNF31 Antibody - BSA Free

Western Blot: HOIP/RNF31 Antibody [NBP1-55059]

Western Blot: HOIP/RNF31 Antibody [NBP1-55059]

Western Blot: HOIP/RNF31 Antibody [NBP1-55059] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Jurkat cell lysate
Western Blot: HOIP/RNF31 Antibody [NBP1-55059]

Western Blot: HOIP/RNF31 Antibody [NBP1-55059]

Western Blot: HOIP/RNF31 Antibody [NBP1-55059] - Lanes: Lane1 : 50 ug hormoxia A549 lysate Lane2: 50 ug hypoxia A549 lysate Lane3: 50 ug hormoxia A549 lysate (+scrambled siRNA) Lane4: 50 ug hypoxia A549 lysate (+scrambled siRNA) Lane5: 50 ug hormoxia A549 lysate (RNF31 siRNA) Lane6: 50 ug hypoxia A549 lysate (RNF31 siRNA) Primary, Antibody Dilution: 1 : 800 Secondary Antibody: Goat anti rabbit HRP Secondary, Antibody Dilution: 1 : 10000 Gene name: RNF31.
Western Blot: HOIP/RNF31 Antibody [NBP1-55059]

Western Blot: HOIP/RNF31 Antibody [NBP1-55059]

Western Blot: HOIP/RNF31 Antibody [NBP1-55059] - Sample Tissue: Human MCF7 Antibody Dilution: 1.0 ug/ml
Western Blot: HOIP/RNF31 Antibody [NBP1-55059]

Western Blot: HOIP/RNF31 Antibody [NBP1-55059]

Western Blot: HOIP/RNF31 Antibody [NBP1-55059] - Sample Type: Jurkat Whole Cell Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 ug/ml Peptide Concentration: 5 ug/ml Lysate Quantity: 25 ug/lane/Lane Gel Concentration: 0.12

Applications for HOIP/RNF31 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HOIP/RNF31

PWP2 contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions.

Long Name

HOIL-1-Interacting Protein

Alternate Names

RNF31, ZIBRA

Gene Symbol

RNF31

UniProt

Q96EP0

Additional HOIP/RNF31 Products

Product Documents for HOIP/RNF31 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HOIP/RNF31 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases
NFkB Pathway

Citations for HOIP/RNF31 Antibody - BSA Free

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail