Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-05457

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Hormone-sensitive Lipase/HSL Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S563 of rat Hormone-sensitive Lipase/HSL (XP_006228456.1). RRSVS

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Modification

p Ser563

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free

Western Blot: Hormone-sensitive Lipase/HSL [p Ser563] Antibody [NBP3-05457] - Western blot analysis of extracts of various cell lines, using Hormone-sensitive Lipase/HSL antibody (NBP3-05457) at 1:1000 dilution.3T3L1 cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.H9C2 cells were treated with Isoproterenol (10 uM for 20 min) at 37C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free

Western Blot: Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free [Hormone-sensitive Lipase/HSL] -

Western Blot: Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free [Hormone-sensitive Lipase/HSL] - Western blot analysis of lysates from Mouse brown fat using Hormone-sensitive Lipase/HSL Rabbit pAb at 1:5000 dilution incubated overnight at 4C. Mouse brown fat were treated by lambda-PP mixed solution (1 ul) at 30C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 30 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Applications for Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Application Notes
Hormone-sensitive Lipase/HSL [p Ser563] antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP3-05457 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Hormone-sensitive Lipase/HSL

Hormone-sensitive lipase (HSL) is an intracellular neutral lipase found in a variety of tissues, but primarily in adipose and steroidogenic tissues. It hydrolyzes triglycerides into free fatty acids and cholesteryl esters into free cholesterol for steroid hormone synthesis.

HSL is the major enzyme responsible for the mobilization of free fatty acids from adopise tissue, and HSL antibodies are useful tools for lipid and metabolism research.

Alternate Names

Hormonesensitive Lipase, HSL, LHS, LIPE

Gene Symbol

LIPE

Additional Hormone-sensitive Lipase/HSL Products

Product Documents for Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Bioactive Lipids and Receptors

Citations for Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free

Customer Reviews for Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free (1)

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Customer Images

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  • Hormone-sensitive Lipase/HSL [p Ser563] Antibody
    Name: Robin Wilson
    Application: Western Blot
    Sample Tested: Differentiated adipocytes
    Species: Mouse
    Verified Customer | Posted 05/05/2021
    Differentiated C3H10T1/2 cells were serum starved and then Treated with CL316243 for 15 minutes. Cells were lysed and lysates were run on a 4-20% gel, transferred to nitrocellulose membranes, blocked for 1hour and the blotted with pHSL (1:1000)
    Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free NBP3-05457
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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