Host Cell Factor 1/HCFC1 Antibody
Novus Biologicals | Catalog # NB100-68210
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Scientific Data Images for Host Cell Factor 1/HCFC1 Antibody
Simple Western: Host Cell Factor 1/HCFC1 Antibody [NB100-68210]
Host-Cell-Factor-1-HCFC1-Antibody-Simple-Western-NB100-68210-img0005.jpgWestern Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] -
Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. a Schematic representation of the affinity purification-mass spectrometry approach to identify the E2F4 interactome. b Silver stain of eluted fractions from RPE cells and mouse ES cells. Slices of gel were subjected to mass spectrometry, & the indicated protein bands were identified by analyzing the proteins enriched in each slice. c GO terms for cellular components enriched in the list of mouse ES cell-specific candidate interactors. d Validation of interactions between GFP-E2F4 & DP-1, HCFC1, YEATS2, LIN54, & LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells & human RPE cells. Transcriptional activators (HCFC1 & YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 & LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31270324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Host Cell Factor 1/HCFC1 Antibody
Immunoprecipitation
Western Blot
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Product Specific Notices for Host Cell Factor 1/HCFC1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Application: Western BlotSample Tested: Mouse Iiver tissue, Sample Amount: 50 ugSpecies: MouseVerified Customer | Posted 11/05/2009
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
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