HOXC11 Antibody (OTI3E10)

Flow Cytometry: HOXC11 Antibody (OTI3E10) [NBP2-00499]

Flow Cytometry: HOXC11 Antibody (3E10) [NBP2-00499] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HOXC11 antibody, and then analyzed by flow cytometry.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499]

Western Blot: HOXC11 Antibody (3E10) [NBP2-00499] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HOXC11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HOXC11.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499]

Western Blot: HOXC11 Antibody (3E10) [NBP2-00499] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using antiHOXC11 monoclonal antibody.(1:200)

Immunohistochemistry-Paraffin: HOXC11 Antibody (OTI3E10) [NBP2-00499]

Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HOXC11 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HOXC11 Antibody (OTI3E10) [NBP2-00499]

Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HOXC11 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HOXC11 Antibody (OTI3E10) [NBP2-00499]

Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HOXC11 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HOXC11 Antibody (OTI3E10) [NBP2-00499]

Immunohistochemistry-Paraffin: HOXC11 Antibody (3E10) [NBP2-00499] - Staining of paraffin-embedded Human endometrium tissue using anti-HOXC11 mouse monoclonal antibody.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

HOXC11 binds to the promoter of SPHK1 to facilitate its expression, predicting a worse prognosis.a GSEA analyzed potential binding targets of HOXC11. b qPCR analysis of CCL5, HBA2, and SPHK1 expression level after stable overexpression of HOXC11. Data are shown as mean +/- SD (n = 3). c Western Blot analysis of SPHK1 protein expression level after HOXC11 stable overexpression. d Western Blot analysis of SPHK1 protein expression level after HOXC11 knockout. e Relative enrichment of SPHK1 promoter in HOXC11 stable overexpressing cells. Data are shown as mean +/- SD (n = 3). f Western Blot detected SPHK1 protein expression of HOXC11 knockout cells and that after transiently transfected with 1.5 μg HOXC11 expression plasmid. NS not significant, *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

HOXC11 is highly expressed in lung adenocarcinoma and correlates with poor overall survival of LUAD.a A heatmap of mRNA level of HOX family genes in LUAD/LUSC and paracancerous samples. b mRNA level of HOXC11 in LUAD/LUSC and paracancerous samples. Data in (a) and (b) are obtained from the TCGA database. c HOXC11 protein expression in LUAD/LUSC and paracancerous tissues. d The relationship between HOXC11 expression and overall survival of lung cancer patients. e The relationship between HOXC11 expression and overall survival of LUAD patients. f The relationship between HOXC11 expression and gender of lung cancer patients. g The relationship between HOXC11 expression and smoking habits of lung cancer patients. Data from (d) to (g) come from the Kaplan–Meier Plotter lung cancer dataset. ***P < 0.001, ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

HOXC11 binds to the promoter of SPHK1 to facilitate its expression, predicting a worse prognosis.a GSEA analyzed potential binding targets of HOXC11. b qPCR analysis of CCL5, HBA2, and SPHK1 expression level after stable overexpression of HOXC11. Data are shown as mean +/- SD (n = 3). c Western Blot analysis of SPHK1 protein expression level after HOXC11 stable overexpression. d Western Blot analysis of SPHK1 protein expression level after HOXC11 knockout. e Relative enrichment of SPHK1 promoter in HOXC11 stable overexpressing cells. Data are shown as mean +/- SD (n = 3). f Western Blot detected SPHK1 protein expression of HOXC11 knockout cells and that after transiently transfected with 1.5 μg HOXC11 expression plasmid. NS not significant, *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

IKK alpha regulates HOXC11 expression at the post-transcriptional level.a Western Blot showed the up-regulation of HOXC11 resulting from IKK alpha stable overexpression. b Western Blot detected the down-regulation of HOXC11 resulting from the IKK alpha knockout. c qPCR analysis of HOXC11 mRNA level after stable overexpression of IKK alpha. Data are shown as mean +/- SD (n = 3). d Western Blot showing HOXC11 protein cumulation after treatment with MG132 (10 μM, 24 h). e Western Blot showed HOXC11 protein degradation after treatment with cycloheximide (10 μg/ml). f Western Blot analysis of HOXC11 accumulation after IKK alpha stable overexpression and MG132 treatment (20 μM, 12 h). g Western Blot showed HOXC11 protein expression after IKK alpha stable overexpression and treatment with cycloheximide (20 μg/ml). h Western Blot analysis of HOXC11 accumulation after IKK alpha knockout and MG132 treatment (20 μM, 10 h). i Western Blot showed HOXC11 protein expression after IKK alpha knockout and treatment with cycloheximide (20 μg/ml). j Ubiquitinating and deubiquitinating enzymes of HOXC11 and IKK alpha predicted by UbiBrowser 2.0. USP8 is shared by HOXC11 and IKK alpha. k USP8 with tag-Myc was instantaneously expressed in HEK293T, and Western Blot detected the protein level of IKK alpha and HOXC11. l Immunofluorescence detected the localization of IKK alpha (red) relative to HOXC11 (green) or Myc-USP8 (green) in PC9 cells. Scale bars, 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

SPHK1 accelerates the progression of lung cancer.a Cell proliferation ability of SPHK1 stable overexpressed cells detected by cell counting kit-8. Data are shown as mean +/- SD (n = 5). b Colony formation assays showing the colony formatted ability of SPHK1 stable overexpressed cells and the quantitative analyses. Data are shown as mean +/- SD (n = 3). c Cell migration and invasion ability of SPHK1 stable overexpressed cells detected by Transwell assays and the quantitative analyses. Data are shown as mean +/- SD (n = 3). d Flow cytometry detecting the cell cycle of SPHK1 overexpressed cells. Differences are compared with the sgCtrl group; data are shown as mean +/- SD (n = 3). e Western blot detected the SPHK1 protein level of HOXC11 overexpressing cells with the treatment of SPHK1 interference. f Cell counting kit-8 detected the cell viability of HOXC11 overexpressing cell line with the treatment of SPHK1 interference. Data are shown as mean +/- SD (n = 5). g Transwell assays detected the migration and invasion ability of HOXC11 overexpressing cell line with the treatment of SPHK1 interference and the quantitative analyses. Differences were compared to the Vector group transfected with Ctrl siRNA. Data are shown as mean +/- SD (n = 3). NS not significant, *P < 0.05, **P < 0.01, *** P < 0.001, ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

HOXC11 binds to the promoter of SPHK1 to facilitate its expression, predicting a worse prognosis.a GSEA analyzed potential binding targets of HOXC11. b qPCR analysis of CCL5, HBA2, and SPHK1 expression level after stable overexpression of HOXC11. Data are shown as mean +/- SD (n = 3). c Western Blot analysis of SPHK1 protein expression level after HOXC11 stable overexpression. d Western Blot analysis of SPHK1 protein expression level after HOXC11 knockout. e Relative enrichment of SPHK1 promoter in HOXC11 stable overexpressing cells. Data are shown as mean +/- SD (n = 3). f Western Blot detected SPHK1 protein expression of HOXC11 knockout cells and that after transiently transfected with 1.5 μg HOXC11 expression plasmid. NS not significant, *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

IKK alpha regulates HOXC11 expression at the post-transcriptional level.a Western Blot showed the up-regulation of HOXC11 resulting from IKK alpha stable overexpression. b Western Blot detected the down-regulation of HOXC11 resulting from the IKK alpha knockout. c qPCR analysis of HOXC11 mRNA level after stable overexpression of IKK alpha. Data are shown as mean +/- SD (n = 3). d Western Blot showing HOXC11 protein cumulation after treatment with MG132 (10 μM, 24 h). e Western Blot showed HOXC11 protein degradation after treatment with cycloheximide (10 μg/ml). f Western Blot analysis of HOXC11 accumulation after IKK alpha stable overexpression and MG132 treatment (20 μM, 12 h). g Western Blot showed HOXC11 protein expression after IKK alpha stable overexpression and treatment with cycloheximide (20 μg/ml). h Western Blot analysis of HOXC11 accumulation after IKK alpha knockout and MG132 treatment (20 μM, 10 h). i Western Blot showed HOXC11 protein expression after IKK alpha knockout and treatment with cycloheximide (20 μg/ml). j Ubiquitinating and deubiquitinating enzymes of HOXC11 and IKK alpha predicted by UbiBrowser 2.0. USP8 is shared by HOXC11 and IKK alpha. k USP8 with tag-Myc was instantaneously expressed in HEK293T, and Western Blot detected the protein level of IKK alpha and HOXC11. l Immunofluorescence detected the localization of IKK alpha (red) relative to HOXC11 (green) or Myc-USP8 (green) in PC9 cells. Scale bars, 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

IKK alpha regulates HOXC11 expression at the post-transcriptional level.a Western Blot showed the up-regulation of HOXC11 resulting from IKK alpha stable overexpression. b Western Blot detected the down-regulation of HOXC11 resulting from the IKK alpha knockout. c qPCR analysis of HOXC11 mRNA level after stable overexpression of IKK alpha. Data are shown as mean +/- SD (n = 3). d Western Blot showing HOXC11 protein cumulation after treatment with MG132 (10 μM, 24 h). e Western Blot showed HOXC11 protein degradation after treatment with cycloheximide (10 μg/ml). f Western Blot analysis of HOXC11 accumulation after IKK alpha stable overexpression and MG132 treatment (20 μM, 12 h). g Western Blot showed HOXC11 protein expression after IKK alpha stable overexpression and treatment with cycloheximide (20 μg/ml). h Western Blot analysis of HOXC11 accumulation after IKK alpha knockout and MG132 treatment (20 μM, 10 h). i Western Blot showed HOXC11 protein expression after IKK alpha knockout and treatment with cycloheximide (20 μg/ml). j Ubiquitinating and deubiquitinating enzymes of HOXC11 and IKK alpha predicted by UbiBrowser 2.0. USP8 is shared by HOXC11 and IKK alpha. k USP8 with tag-Myc was instantaneously expressed in HEK293T, and Western Blot detected the protein level of IKK alpha and HOXC11. l Immunofluorescence detected the localization of IKK alpha (red) relative to HOXC11 (green) or Myc-USP8 (green) in PC9 cells. Scale bars, 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

IKK alpha regulates HOXC11 expression at the post-transcriptional level.a Western Blot showed the up-regulation of HOXC11 resulting from IKK alpha stable overexpression. b Western Blot detected the down-regulation of HOXC11 resulting from the IKK alpha knockout. c qPCR analysis of HOXC11 mRNA level after stable overexpression of IKK alpha. Data are shown as mean +/- SD (n = 3). d Western Blot showing HOXC11 protein cumulation after treatment with MG132 (10 μM, 24 h). e Western Blot showed HOXC11 protein degradation after treatment with cycloheximide (10 μg/ml). f Western Blot analysis of HOXC11 accumulation after IKK alpha stable overexpression and MG132 treatment (20 μM, 12 h). g Western Blot showed HOXC11 protein expression after IKK alpha stable overexpression and treatment with cycloheximide (20 μg/ml). h Western Blot analysis of HOXC11 accumulation after IKK alpha knockout and MG132 treatment (20 μM, 10 h). i Western Blot showed HOXC11 protein expression after IKK alpha knockout and treatment with cycloheximide (20 μg/ml). j Ubiquitinating and deubiquitinating enzymes of HOXC11 and IKK alpha predicted by UbiBrowser 2.0. USP8 is shared by HOXC11 and IKK alpha. k USP8 with tag-Myc was instantaneously expressed in HEK293T, and Western Blot detected the protein level of IKK alpha and HOXC11. l Immunofluorescence detected the localization of IKK alpha (red) relative to HOXC11 (green) or Myc-USP8 (green) in PC9 cells. Scale bars, 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

IKK alpha regulates HOXC11 expression at the post-transcriptional level.a Western Blot showed the up-regulation of HOXC11 resulting from IKK alpha stable overexpression. b Western Blot detected the down-regulation of HOXC11 resulting from the IKK alpha knockout. c qPCR analysis of HOXC11 mRNA level after stable overexpression of IKK alpha. Data are shown as mean +/- SD (n = 3). d Western Blot showing HOXC11 protein cumulation after treatment with MG132 (10 μM, 24 h). e Western Blot showed HOXC11 protein degradation after treatment with cycloheximide (10 μg/ml). f Western Blot analysis of HOXC11 accumulation after IKK alpha stable overexpression and MG132 treatment (20 μM, 12 h). g Western Blot showed HOXC11 protein expression after IKK alpha stable overexpression and treatment with cycloheximide (20 μg/ml). h Western Blot analysis of HOXC11 accumulation after IKK alpha knockout and MG132 treatment (20 μM, 10 h). i Western Blot showed HOXC11 protein expression after IKK alpha knockout and treatment with cycloheximide (20 μg/ml). j Ubiquitinating and deubiquitinating enzymes of HOXC11 and IKK alpha predicted by UbiBrowser 2.0. USP8 is shared by HOXC11 and IKK alpha. k USP8 with tag-Myc was instantaneously expressed in HEK293T, and Western Blot detected the protein level of IKK alpha and HOXC11. l Immunofluorescence detected the localization of IKK alpha (red) relative to HOXC11 (green) or Myc-USP8 (green) in PC9 cells. Scale bars, 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

IKK alpha regulates HOXC11 expression at the post-transcriptional level.a Western Blot showed the up-regulation of HOXC11 resulting from IKK alpha stable overexpression. b Western Blot detected the down-regulation of HOXC11 resulting from the IKK alpha knockout. c qPCR analysis of HOXC11 mRNA level after stable overexpression of IKK alpha. Data are shown as mean +/- SD (n = 3). d Western Blot showing HOXC11 protein cumulation after treatment with MG132 (10 μM, 24 h). e Western Blot showed HOXC11 protein degradation after treatment with cycloheximide (10 μg/ml). f Western Blot analysis of HOXC11 accumulation after IKK alpha stable overexpression and MG132 treatment (20 μM, 12 h). g Western Blot showed HOXC11 protein expression after IKK alpha stable overexpression and treatment with cycloheximide (20 μg/ml). h Western Blot analysis of HOXC11 accumulation after IKK alpha knockout and MG132 treatment (20 μM, 10 h). i Western Blot showed HOXC11 protein expression after IKK alpha knockout and treatment with cycloheximide (20 μg/ml). j Ubiquitinating and deubiquitinating enzymes of HOXC11 and IKK alpha predicted by UbiBrowser 2.0. USP8 is shared by HOXC11 and IKK alpha. k USP8 with tag-Myc was instantaneously expressed in HEK293T, and Western Blot detected the protein level of IKK alpha and HOXC11. l Immunofluorescence detected the localization of IKK alpha (red) relative to HOXC11 (green) or Myc-USP8 (green) in PC9 cells. Scale bars, 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

IKK alpha regulates HOXC11 expression at the post-transcriptional level.a Western Blot showed the up-regulation of HOXC11 resulting from IKK alpha stable overexpression. b Western Blot detected the down-regulation of HOXC11 resulting from the IKK alpha knockout. c qPCR analysis of HOXC11 mRNA level after stable overexpression of IKK alpha. Data are shown as mean +/- SD (n = 3). d Western Blot showing HOXC11 protein cumulation after treatment with MG132 (10 μM, 24 h). e Western Blot showed HOXC11 protein degradation after treatment with cycloheximide (10 μg/ml). f Western Blot analysis of HOXC11 accumulation after IKK alpha stable overexpression and MG132 treatment (20 μM, 12 h). g Western Blot showed HOXC11 protein expression after IKK alpha stable overexpression and treatment with cycloheximide (20 μg/ml). h Western Blot analysis of HOXC11 accumulation after IKK alpha knockout and MG132 treatment (20 μM, 10 h). i Western Blot showed HOXC11 protein expression after IKK alpha knockout and treatment with cycloheximide (20 μg/ml). j Ubiquitinating and deubiquitinating enzymes of HOXC11 and IKK alpha predicted by UbiBrowser 2.0. USP8 is shared by HOXC11 and IKK alpha. k USP8 with tag-Myc was instantaneously expressed in HEK293T, and Western Blot detected the protein level of IKK alpha and HOXC11. l Immunofluorescence detected the localization of IKK alpha (red) relative to HOXC11 (green) or Myc-USP8 (green) in PC9 cells. Scale bars, 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

IKK alpha regulates HOXC11 expression at the post-transcriptional level.a Western Blot showed the up-regulation of HOXC11 resulting from IKK alpha stable overexpression. b Western Blot detected the down-regulation of HOXC11 resulting from the IKK alpha knockout. c qPCR analysis of HOXC11 mRNA level after stable overexpression of IKK alpha. Data are shown as mean +/- SD (n = 3). d Western Blot showing HOXC11 protein cumulation after treatment with MG132 (10 μM, 24 h). e Western Blot showed HOXC11 protein degradation after treatment with cycloheximide (10 μg/ml). f Western Blot analysis of HOXC11 accumulation after IKK alpha stable overexpression and MG132 treatment (20 μM, 12 h). g Western Blot showed HOXC11 protein expression after IKK alpha stable overexpression and treatment with cycloheximide (20 μg/ml). h Western Blot analysis of HOXC11 accumulation after IKK alpha knockout and MG132 treatment (20 μM, 10 h). i Western Blot showed HOXC11 protein expression after IKK alpha knockout and treatment with cycloheximide (20 μg/ml). j Ubiquitinating and deubiquitinating enzymes of HOXC11 and IKK alpha predicted by UbiBrowser 2.0. USP8 is shared by HOXC11 and IKK alpha. k USP8 with tag-Myc was instantaneously expressed in HEK293T, and Western Blot detected the protein level of IKK alpha and HOXC11. l Immunofluorescence detected the localization of IKK alpha (red) relative to HOXC11 (green) or Myc-USP8 (green) in PC9 cells. Scale bars, 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: HOXC11 Antibody (OTI3E10) [NBP2-00499] -

IKK alpha regulates HOXC11 expression at the post-transcriptional level.a Western Blot showed the up-regulation of HOXC11 resulting from IKK alpha stable overexpression. b Western Blot detected the down-regulation of HOXC11 resulting from the IKK alpha knockout. c qPCR analysis of HOXC11 mRNA level after stable overexpression of IKK alpha. Data are shown as mean +/- SD (n = 3). d Western Blot showing HOXC11 protein cumulation after treatment with MG132 (10 μM, 24 h). e Western Blot showed HOXC11 protein degradation after treatment with cycloheximide (10 μg/ml). f Western Blot analysis of HOXC11 accumulation after IKK alpha stable overexpression and MG132 treatment (20 μM, 12 h). g Western Blot showed HOXC11 protein expression after IKK alpha stable overexpression and treatment with cycloheximide (20 μg/ml). h Western Blot analysis of HOXC11 accumulation after IKK alpha knockout and MG132 treatment (20 μM, 10 h). i Western Blot showed HOXC11 protein expression after IKK alpha knockout and treatment with cycloheximide (20 μg/ml). j Ubiquitinating and deubiquitinating enzymes of HOXC11 and IKK alpha predicted by UbiBrowser 2.0. USP8 is shared by HOXC11 and IKK alpha. k USP8 with tag-Myc was instantaneously expressed in HEK293T, and Western Blot detected the protein level of IKK alpha and HOXC11. l Immunofluorescence detected the localization of IKK alpha (red) relative to HOXC11 (green) or Myc-USP8 (green) in PC9 cells. Scale bars, 20 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36823149), licensed under a CC-BY license. Not internally tested by Novus Biologicals.