HP1 Alpha, Beta, and Gamma Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-59154

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HP1 Alpha, Beta, and Gamma Primary Antibodies »

Product Specifications

Immunogen

The exact immunogen is propietary information.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HP1 Alpha, Beta, and Gamma Antibody - BSA Free

Western Blot: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154]

Western Blot: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154]

Western Blot: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - Western blot was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1alpha,beta and gamma diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (kDa) is shown on the left; the expected location of HP1alpha, HP1beta and HP1gamma is indicated on the right.
Chromatin Immunoprecipitation: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - ChIP assays were performed using NIH3T3 cells and 4 ug of the antibody directed against HP1alpha,beta and gamma. QPCR was performed on the IP'd DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. Figure shows the relative enrichment as compared to a no antibody negative control ChIP.
Immunofluorescence: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154]

Immunofluorescence: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154]

Immunofluorescence: HP1 Alpha, Beta, and Gamma Antibody [NBP2-59154] - HeLa cells were stained with the antibody against HP1alpha, beta and gamma; and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1alpha,beta and gamma antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Applications for HP1 Alpha, Beta, and Gamma Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

4 ug/IP

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide and 0.05% ProClin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HP1 Alpha, Beta, and Gamma

Alternate Names

Antigen p25, CBXM31HP1-BETA, chromobox homolog 1, chromobox homolog 1 (Drosophila HP1 beta), chromobox homolog 1 (HP1 beta homolog Drosophila ), chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox homolog 5, chromobox homolog 5 (Drosophila HP1 alpha), chromobox homolog 5 (HP1 alpha homolog, Drosophila), chromobox protein homolog 1, chromobox protein homolog 3, chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha, Heterochromatin protein 1 homolog beta, Heterochromatin protein 1 homolog gamma, heterochromatin protein 1-beta, heterochromatin protein HP1 gamma, Heterochromatin protein p25, heterochromatin protein p25 beta, heterochromatin-like protein 1, HP1, HP1 alpha, HP1 alpha homolog, HP1 beta, HP1 beta homolog, HP1 gamma, HP1A, HP1-ALPHA, HP1-GAMMA, HP1Hs alpha, HP1Hs-alpha, HP1Hsbeta, HP1Hs-beta, MOD1, Modifier 1 protein, Modifier 2 protein, p25beta

Gene Symbol

CBX5

Additional HP1 Alpha, Beta, and Gamma Products

Product Documents for HP1 Alpha, Beta, and Gamma Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HP1 Alpha, Beta, and Gamma Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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