HPa3 Antibody (HIC3-2D12) - BSA Free
Novus Biologicals | Catalog # NBP3-18520
![Immunohistochemistry-Paraffin: HPa3 Antibody (HIC3-2D12) [NBP3-18520]](https://resources.rndsystems.com/images/products/HPa3-Antibody-HIC3-2D12-Immunohistochemistry-Paraffin-NBP3-18520-img0001.jpg "Immunohistochemistry-Paraffin: HPa3 Antibody (HIC3-2D12) [NBP3-18520]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # HIC3-2D12
Format
BSA Free
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Product Specifications
Immunogen
Human pancreatic islets enriched in non-beta endocine cells.
Specificity
This HPa3 antibody does not label beta cells, labels delta cells dimly, and brightly labels alpha, gamma, and epsilon cells in human pancreatic islets.
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for HPa3 Antibody (HIC3-2D12) - BSA Free
Immunohistochemistry-Paraffin: HPa3 Antibody (HIC3-2D12) [NBP3-18520]
Immunohistochemistry-Paraffin: HPa3 Antibody (HIC3-2D12) [NBP3-18520] - Formalin Fixed Paraffin Embedded (FFPE) human pancreatic tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:50 dilution of Cat. No. MABS1998, Anti-HPa3, clone HIC3-2D12. Reactivity was detected using an Rabbit Anti-Mouse and DAB. Cytoplasmic/membranous staining in islets of human pancreas (Left image). membranous staining in intercalated ducts (Middle image), as well as intralobular ducts of human pancreas (Right image) tissues was observed.Applications for HPa3 Antibody (HIC3-2D12) - BSA Free
Application
Recommended Usage
Flow Cytometry
reported in scientific literature (PMID 27399229)
Immunohistochemistry
1:50
Immunohistochemistry-Paraffin
1:50. Use reported in scientific literature (PMID 27399229)
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Ion exchange chromatography
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
2 mg/ml
Stability & Storage
Store at 4°C.
Background: HPa3
Additional HPa3 Products
Product Documents for HPa3 Antibody (HIC3-2D12) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HPa3 Antibody (HIC3-2D12) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for HPa3 Antibody (HIC3-2D12) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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