HPi2 Antibody (HIC1-2B4.2B) - BSA Free

Novus Biologicals | Catalog # NBP1-18946

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HIC1-2B4.2B

Format

BSA Free
View all HPi2 Primary Antibodies »

Product Specifications

Immunogen

Human pancreatic enriched islet cells containing low levels of exocrine and ductal cells.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26515645).

Localization

Cell surface

Marker

Endocrine pan-islet cell Marker

Specificity

Specific for multiple endocrine cell types.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HPi2 Antibody (HIC1-2B4.2B) - BSA Free

Immunocytochemistry/ Immunofluorescence: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946]

Immunocytochemistry/ Immunofluorescence: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946]

Immunocytochemistry/Immunofluorescence: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946] - Immunofluorescence on frozen section of human pancreas.
Flow Cytometry: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946]

Flow Cytometry: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946]

Flow Cytometry: HPi2 Antibody (HIC1-2B4.2B) [NBP1-18946] - Analysis of enzyme dispersed human pancreas cells.

Applications for HPi2 Antibody (HIC1-2B4.2B) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100
Application Notes
This HPi2 (HIC1-2B4.2B) antibody is useful for Immunohistochemistry on acetone fixed frozen sections, Immunocytochemistry/Immunofluorescence and Flow cytometry.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue culture supernatant

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HPi2

Stem cells offer a therapeutic promise for many diseases. In the case of diabetes, stem cells offer the promise that beta cells may be generated ex vivo for the possible transplantation and cure of Type I diabetes (Couri CE, et al.). For this to happen, stem cells will need to be isolated and expanded, they will need to be differentiated toward the beta cell lineage, and the differentiated cell progeny used for transplantation may need to be isolated from complex cell mixtures following differentiation. Appropriate markers targeting cell surface molecules on developing and mature pancreatic cells will be required for such research.

Alternate Names

human pancreatic endocrine islet cell

Additional HPi2 Products

Product Documents for HPi2 Antibody (HIC1-2B4.2B) - BSA Free

Certificate of Analysis

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Product Specific Notices for HPi2 Antibody (HIC1-2B4.2B) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HPi2 Antibody (HIC1-2B4.2B) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for HPi2 Antibody (HIC1-2B4.2B) - BSA Free

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  • Q: I would be interested by the Test samples for HPi2 and HPa2 antibodies. I would like to use them for FACS and combine them to dissociate human pancreatic alpha-cells and beta-cells as described in Dorrell C et al Diabetologia 2011. However according to your website, both NBP1-18946 and NBP1-18950 seem to be Mouse IgG1, whereas it is stated in ESM Table 2 of this paper that HPa2 would be an IgM. Could you please explain me this discrepancy ?

    A: I can confirm that our HPa2 Ab is IgG1. Our Hpi2 antibody is also IgG1. I checked these in our internal database.

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