HPRT Antibody (24GB6185)

Novus Biologicals | Catalog # NBP3-51023

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB6185
View all HPRT Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human HPRT (Uniprot: P00492)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HPRT Antibody (24GB6185)

HPRT Antibody (24GB6185)

Immunocytochemistry/ Immunofluorescence: HPRT Antibody (24GB6185)[NBP3-51023] -

Immunocytochemical staining of HepG2 cells with HPRT antibody (1:1,000). Nuclei were stained blue with DAPI; HPRT was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
HPRT Antibody (24GB6185)

Flow Cytometry: HPRT Antibody (24GB6185)[NBP3-51023] -

Validation of HPRT knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HPRT antibody (1:2,000) and analyzed using BD flow cytometer.
HPRT Antibody (24GB6185)

Flow Cytometry: HPRT Antibody (24GB6185)[NBP3-51023] -

Flow cytometric analysis of HPRTexpression in HepG2 cells using HPRT antibody (1:2,000). Green, isotype control; red, HPRT1.
HPRT Antibody (24GB6185)

Western Blot: HPRT Antibody (24GB6185)[NBP3-51023] -

Western blotting analysis using HPRT antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
HPRT Antibody (24GB6185)

Western Blot: HPRT Antibody (24GB6185)[NBP3-51023] -

Western blotting analysis using HPRT antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for HPRT Antibody (24GB6185)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HPRT

HPRT is encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Long Name

Hypoxanthine-Guanine Phosphoribosyltransferase

Alternate Names

HGPRT, HGPRT1, HPRT1

Gene Symbol

HPRT1

Additional HPRT Products

Product Documents for HPRT Antibody (24GB6185)

Certificate of Analysis

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Product Specific Notices for HPRT Antibody (24GB6185)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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