HPRT Antibody (JU03-26)
Novus Biologicals | Catalog # NBP2-75528
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]](https://resources.rndsystems.com/images/products/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0004.jpg "Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JU03-26 expressed in HEK293
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Product Specifications
Immunogen
Synthetic peptide within Human HPRT aa 169-218 / 218. (SwissProt: P00492 Human; SwissProt: P00493 Mouse; SwissProt: P27605 Rat)
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HPRT Antibody (JU03-26)
Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]
Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]](https://resources.rndsystems.com/images/products/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0001.jpg "Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]")
Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]
Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]](https://resources.rndsystems.com/images/products/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0002.jpg "Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]")
Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]
Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]](https://resources.rndsystems.com/images/products/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0003.jpg "Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]")
Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]
Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human kidney tissue using anti-HPRT antibody. Counter stained with hematoxylin.Applications for HPRT Antibody (JU03-26)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Application Notes
IP-Assay dependent
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HPRT
Long Name
Hypoxanthine-Guanine Phosphoribosyltransferase
Alternate Names
HGPRT, HGPRT1, HPRT1
Gene Symbol
HPRT1
Additional HPRT Products
Product Documents for HPRT Antibody (JU03-26)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HPRT Antibody (JU03-26)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for HPRT Antibody (JU03-26)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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