HR6B/UBE2B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-58772

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HR6B/UBE2B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HR6B/UBE2B Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HR6B/UBE2B Antibody [NBP2-58772]

Immunocytochemistry/ Immunofluorescence: HR6B/UBE2B Antibody [NBP2-58772]

Immunocytochemistry/Immunofluorescence: HR6B/UBE2B Antibody [NBP2-58772] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol.
HR6B/UBE2B Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: HR6B/UBE2B Antibody - BSA Free [NBP2-58772]

Chromatin Immunoprecipitation-exo-Seq: HR6B/UBE2B Antibody - BSA Free [NBP2-58772]

ChIP-Exo-Seq composite graph for Anti-UBE2B (NBP2-58772) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for HR6B/UBE2B Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HR6B/UBE2B

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]

Long Name

Ubiquitin-conjugating Enzyme E2B

Alternate Names

E2-17k, UBE2B

Gene Symbol

UBE2B

Additional HR6B/UBE2B Products

Product Documents for HR6B/UBE2B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HR6B/UBE2B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
p53 Pathway

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail