HSF1 Antibody (4B4) [DyLight 650]

Novus Biologicals | Catalog # NBP2-42206C

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit

Applications

Immunohistochemistry, ELISA, Immunoprecipitation, Gel Super Shift Assays

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 4B4
View all HSF1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Human, Rat, Bovine, Guinea Pig, Hamster, Monkey and Rabbit.

Localization

Cytoplasm, Nucleus

Specificity

Detects approx 85kDa (unstressed cell lysates) and approx 95kDa (heat shocked cell lysates).

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for HSF1 Antibody (4B4) [DyLight 650]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Gel Super Shift Assays

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HSF1

Human cells respond to heat stress by inducing the binding of a pre-existing transcriptional activator (heat shock factor, HSF) to DNA (1). Induction of heat shock protein (HSP) gene expression by stress is initiated by binding of HSF1 to HSP gene promoters to increase their transcription. The cytoprotective functions of these HSPs are essential for cell survival, and thus it is critical that inducible HSP gene expression be executed rapidly and efficiently. There is an interaction between heat shock factor 1 (HSF1) and symplekin, a protein known to form a complex with the polyadenylation factors CstF and CPSF. HSF1-symplekin complexes are detected only after stress treatment, and these two proteins co-localize in punctate nuclear structures in stressed cells (2). A chaperone/Hsp functioning as repressor of heat shock transcription factor (HSF) could make activation of hsp genes dependent on protein unfolding. It has been concluded that Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1 (3).

Long Name

Heat Shock Transcription Factor 1

Alternate Names

HSTF1

Gene Symbol

HSF1

Additional HSF1 Products

Product Documents for HSF1 Antibody (4B4) [DyLight 650]

Certificate of Analysis

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Product Specific Notices for HSF1 Antibody (4B4) [DyLight 650]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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