Hsp47 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16893

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (96%), Canine (98%), Porcine (96%), Primate (100%), Rat (97%), Rhesus Macaque (100%), Sheep (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSP47 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Hsp47. The exact sequence is proprietary.

Reactivity Notes

Chicken (88%).

Localization

Endoplasmic reticulum lumen

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Hsp47 Antibody - BSA Free

Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893]

Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893]

Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893] - Sample: Paraffin-embedded mouse kidney. Hsp47 antibody Internal dilution: 1:500.
Western Blot: Hsp47 Antibody [NBP2-16893]

Western Blot: Hsp47 Antibody [NBP2-16893]

Western Blot: Hsp47 Antibody [NBP2-16893] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893]

Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893]

Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893] - Sample: Paraffin-embedded human breast carcinoma. Hsp47 antibody Internal dilution: 1:500.
Hsp47 Antibody - BSA Free

Western Blot: Hsp47 Antibody [NBP2-16893] -

HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.
A. 30 ug U87-MG whole cell lysate/extract
B. 30 ug SK-N-SH whole cell lysate/extract
C. 30 ug IMR32 whole cell lysate/extract
D. 30 ug SK-N-AS whole cell lysate/extract
10% SDS-PAGE
HSP47 antibody [N2C2], Internal (NBP2-16893) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Hsp47 Antibody - BSA Free

Western Blot: Hsp47 Antibody [NBP2-16893] -

HSP47 antibody [N2C2], Internal detects HSP47 protein by western blot analysis.
A. 30 ug A431 whole cell lysate/extract
B. 30 ug HeLa whole cell lysate/extract
C. 30 ug HepG2 whole cell lysate/extract
10% SDS-PAGE
HSP47 antibody [N2C2], Internal (NBP2-16893) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Hsp47 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSP47

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. [provided by RefSeq]

Long Name

Heat Shock Protein 47

Alternate Names

AsTP3, BERF-1, CBP1, CBP2, Colligin, gp46, OI10, PPROM, RA-A47, Serpin H1, SERPINH1

Gene Symbol

SERPINH1

UniProt

P50454

Additional HSP47 Products

Product Documents for Hsp47 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Hsp47 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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