HSP70/HSPA1A Antibody (5U9A0)

Novus Biologicals | Catalog # NBP3-16482

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5U9A0 expressed in HEK293
View all HSP70/HSPA1A Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 441-641 of human HSP70/HSPA1A (P0DMV8). QVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP70/HSPA1A Antibody (5U9A0)

Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]

Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]

Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Western blot analysis of extracts of various cell lines, using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]

Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Immunofluorescence analysis of NIH-3T3 cells using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]

Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Immunofluorescence analysis of HeLa cells using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for HSP70/HSPA1A Antibody (5U9A0)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody (5U9A0)

Certificate of Analysis

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Product Specific Notices for HSP70/HSPA1A Antibody (5U9A0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail