HSP70/HSPA1A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77455

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Primate (100%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSP70/HSPA1A Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human Hsp70 protein (between residues 550-600) [Uniprot: P08107]

Localization

Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP70/HSPA1A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody [NBP1-77455] - HSP70 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Immunohistochemistry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77455] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.
Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on Jurkat cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - Single cell suspension generated from B16-F10 tumor excised from C57BL/6 Mouse stained with Hsp70 & FITC secondary (blue) or unstained (red). [Image provided by a varified research professional; see Product Reviews for details on protocol employed].
Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on A549 cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Simple Western: HSP70/HSPA1A AntibodyBSA Free [NBP1-77455]

Simple Western: HSP70/HSPA1A AntibodyBSA Free [NBP1-77455]

Simple Western: HSP70/HSPA1A Antibody [NBP1-77455] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for HSP70/HSPA1A Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1 : 200

Immunocytochemistry/ Immunofluorescence

1:25

Immunohistochemistry

1:400

Immunohistochemistry-Frozen

reported in scientific literature (PMID 26755821)

Immunohistochemistry-Paraffin

1:400

Simple Western

1:50
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 67 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 3 using NBP1-77455 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.18 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

Heat shock protein 70 1A/1B (also known as HSP70, HSPA1A, HSPA1B, HSP70 1/2) is a chaperone protein which can stabilize proteins against aggregation and assist in protein folding. It has been shown to play a role in aging, viral replication, lupus, and tumor progression. Accordingly, HSP70 has been used in anti-tumor vaccines, as well as HIV and Parkinson's research.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

UniProt

P08107

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HSP70/HSPA1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HSP70/HSPA1A Antibody - BSA Free

Customer Reviews for HSP70/HSPA1A Antibody - BSA Free (1)

3 out of 5
1 Customer Rating
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Customer Images

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  • Name: Subhash Gangar
    Application: Flow Cytometry
    Sample Tested: Mouse Melanoma B16-F10
    Species: Mouse
    Verified Customer | Posted 08/20/2014
    B16-F10 Tumor Excised from C57BL/6 Mouse stained with Hsp70 and FITC secondary (blue) or unstained (red)
    HSP70/HSPA1A Antibody - BSA Free NBP1-77455

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Protocols

View specific protocols for HSP70/HSPA1A Antibody - BSA Free (NBP1-77455):

HSP70/HSPA1A Antibody:
Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

HSP70/HSPA1A Antibody:
Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail