HSP90 Antibody (16F1) - BSA Free
Novus Biologicals | Catalog # NBP1-97506
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Scientific Data Images for HSP90 Antibody (16F1) - BSA Free
Western Blot: HSP90 Antibody (16F1) [NBP1-97506]
Western Blot: HSP90 Antibody (16F1) [NBP1-97506] - Lane 1: MW marker, Lane 2: HSP90 native protein, Lane 3: HSP90b recombinant protein, Lane 4: HSP90alpha recombinant protein, Lane 5: HeLa (HS), Lane 6: L-929 (HS), Lane 7: Rat-2 (HS), Lane 8:RK-13 (HS).Immunohistochemistry-Paraffin: HSP90 Antibody (16F1) [NBP1-97506]
Immunohistochemistry-Paraffin: HSP90 Antibody (16F1) [NBP1-97506] - Analysis of human spleen tissue stained with HSP90, mAb (16F1) at 10ug/ml.Western Blot: HSP90 Antibody (16F1) - BSA Free [NBP1-97506] -
Ixazomib increases heat-shock proteins (HSPs) in mouse Schwann cellsImmortalized mouse Schwann cells treated with either 10 μg/mL Ixazomib (Ixa) or dimethyl sulfoxide (DMSO) for 24 h were subjected to isobaric tags for relative and absolute quantitation-mass spectrometry (iTRAQ-MS) proteomics.(A) Overview of the 66 significantly changed proteins (false discovery rate [FDR]-corrected p < 0.05) in four Ixazomib (Ixa) groups as compared with three DMSO groups. The heatmap represents the log2 (fold change [FC]) of the differentially expressed proteins in each group. The color key from blue to red represents the log2 (FC) from low to high. See Tables S3A and S3B for details.(B) Enrichment analysis of differentially expressed proteins. The vertical axis represents the pathway category and the horizontal axis represents the enrichment score [–log10 (P)] of the pathway. See Table S3C for details.(C) Volcano plot representing log10 (FDR) as a function of log2 (FC). The proteins identified with an FDR-value <0.05 and >1.0-fold are highlighted in red (up-regulated) or blue (down-regulated), with the dotted lines representing the boundary for identification.(D) Immunoblotting analyses using antibodies against poly-ubiquitin (polyUb), HSP40, HSP70, HSP90, and proteasome modulator 9 (PSMD9).(E) Quantification of relative protein amounts in (D). beta -Actin was used for internal control. Unpaired t test. Data are represented As means +/- SEM. ns, not significant, ∗p < 0.05, ∗∗p < 0.01, N = 3 for untreated (DMSO), and 4 for Ixazomib. Image collected and cropped by CiteAb from the following open publication (https://linkinghub.elsevier.com/retrieve/pii/S258900422301074X), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for HSP90 Antibody (16F1) - BSA Free
Immunohistochemistry
Immunohistochemistry-Paraffin
Immunoprecipitation
Western Blot
Formulation, Preparation, and Storage
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Background: HSP90
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Product Documents for HSP90 Antibody (16F1) - BSA Free
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Product Specific Notices for HSP90 Antibody (16F1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars