HSP90 beta Antibody (23GB2785)

Novus Biologicals | Catalog # NBP3-51143

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2785
View all HSP90 beta Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human HSP90 beta (Uniprot: P08238)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP90 beta Antibody (23GB2785)

HSP90 beta Antibody (23GB2785)

Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody (23GB2785)[NBP3-51143] -

Immunocytochemical staining of HepG2 cells with HSP90 beta antibody (1:1,000). Nuclei were stained blue with DAPI; HSP90 beta was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
HSP90 beta Antibody (23GB2785)

Flow Cytometry: HSP90 beta Antibody (23GB2785)[NBP3-51143] -

Validation of HSP90 beta knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) 293T cells were stained with HSP90 beta antibody (1:2,000) and analyzed using BD flow cytometer.
HSP90 beta Antibody (23GB2785)

Flow Cytometry: HSP90 beta Antibody (23GB2785)[NBP3-51143] -

Flow cytometric analysis of HSP90 betaexpression in HepG2 cells using HSP90 beta antibody (1:2,000). Green, isotype control; red, Hsp90 beta.

Applications for HSP90 beta Antibody (23GB2785)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP90 beta

The 90-kDa heat shock protein (Hsp90) is a highly conserved and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine, serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform, (Hsp90-

Long Name

Heat Shock Protein 90 beta

Alternate Names

D6S182, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB

Gene Symbol

HSP90AB1

Additional HSP90 beta Products

Product Documents for HSP90 beta Antibody (23GB2785)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HSP90 beta Antibody (23GB2785)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

Apoptosis Signaling Pathway
Apoptosis Signaling Pathway Thumbnail