HSP90 beta Antibody (HSP90AB1/3953)
Novus Biologicals | Catalog # NBP3-07192
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # HSP90AB1/3953
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Product Specifications
Immunogen
Recombinant human HSP90 beta protein fragment (around aa581-704) (exact sequence is proprietary) (Uniprot: P08238)
Localization
Cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-08908)
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for HSP90 beta Antibody (HSP90AB1/3953)
![Western Blot: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]](https://resources.rndsystems.com/images/products/HSP90-beta-Antibody-HSP90AB1-3953-Western-Blot-NBP3-07192-img0004.jpg "Western Blot: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]")
Western Blot: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]
Western Blot: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192] - Western blot analysis of MCF-7 cell lysate using HSP90 beta antibody (HSP90AB1/3953).![Immunohistochemistry-Paraffin: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]](https://resources.rndsystems.com/images/products/HSP90-beta-Antibody-HSP90AB1-3953-Immunohistochemistry-Paraffin-NBP3-07192-img0002.jpg "Immunohistochemistry-Paraffin: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]")
Immunohistochemistry-Paraffin: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]
Immunohistochemistry-Paraffin: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192] - Formalin-fixed, paraffin-embedded human prostate stained with HSP90 beta Mouse Monoclonal Antibody (HSP90 beta/3953).![Protein Array: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]](https://resources.rndsystems.com/images/products/HSP90-beta-Antibody-HSP90AB1-3953-Protein-Array-NBP3-07192-img0001.jpg "Protein Array: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]")
Protein Array: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]
Protein Array: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HSP90 beta Mouse Monoclonal Antibody (HSP90 beta/3953).Applications for HSP90 beta Antibody (HSP90AB1/3953)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: HSP90 beta
Long Name
Heat Shock Protein 90 beta
Alternate Names
D6S182, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB
Gene Symbol
HSP90AB1
UniProt
Additional HSP90 beta Products
Product Documents for HSP90 beta Antibody (HSP90AB1/3953)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HSP90 beta Antibody (HSP90AB1/3953)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
