HSPA2 Antibody (5C9X0)

Novus Biologicals | Catalog # NBP3-16638

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5C9X0 expressed in HEK293
View all HSPA2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 488-637 of human HSPA2 (P54652). ILNVTAADKSTGKENKITITNDKGRLSKDDIDRMVQEAERYKSEDEANRDRVAAKNALESYTYNIKQTVEDEKLRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEYEHKQKELERVCNPIISKLYQGGPGGGSGGGGSGASGGPTIEE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSPA2 Antibody (5C9X0)

Western Blot: HSPA2 Antibody (5C9X0) [NBP3-16638]

Western Blot: HSPA2 Antibody (5C9X0) [NBP3-16638]

Western Blot: HSPA2 Antibody (5C9X0) [NBP3-16638] - Western blot analysis of extracts of various cell lines, using HSPA2 Rabbit mAb (NBP3-16638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: HSPA2 Antibody (5C9X0) [NBP3-16638]

Immunocytochemistry/ Immunofluorescence: HSPA2 Antibody (5C9X0) [NBP3-16638]

Immunocytochemistry/Immunofluorescence: HSPA2 Antibody (5C9X0) [NBP3-16638] - Immunofluorescence analysis of U-2 OS cells using HSPA2 Rabbit mAb (NBP3-16638) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: HSPA2 Antibody (5C9X0) [NBP3-16638]

Western Blot: HSPA2 Antibody (5C9X0) [NBP3-16638]

Western Blot: HSPA2 Antibody (5C9X0) [NBP3-16638] - Western blot analysis of extracts of various cell lines, using HSPA2 Rabbit mAb (NBP3-16638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
HSPA2 Antibody (5C9X0)

Immunocytochemistry/ Immunofluorescence: HSPA2 Antibody (5C9X0) [NBP3-16638] -

Immunocytochemistry/ Immunofluorescence: HSPA2 Antibody (5C9X0) [NBP3-16638] - Confocal imaging of paraffin-embedded Rat testis tissue using HSPA2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for HSPA2 Antibody (5C9X0)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSPA2

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediatethe folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participatein all these processes through their ability to recognize nonnative conformations of other proteins. They bindextended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membranetranslocation, or following stress-induced damage

Long Name

Heat Shock Protein A2

Alternate Names

HSP70-2

Gene Symbol

HSPA2

Additional HSPA2 Products

Product Documents for HSPA2 Antibody (5C9X0)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for HSPA2 Antibody (5C9X0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Heat Shock Proteins (HSPs)

Customer Reviews for HSPA2 Antibody (5C9X0)

There are currently no reviews for this product. Be the first to review HSPA2 Antibody (5C9X0) and earn rewards!

Have you used HSPA2 Antibody (5C9X0)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies