HspA6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85949

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HSPA6/HSP70B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVERMVHEAEQYKAEDEAQRDRVAAKNSLEAHVFHVKGSLQEESLRDKIPEEDRRKMQDKCREVLAWLEHNQLAEKEEYEHQKRELEQICRPIFSRLYG

Reactivity Notes

Reported reactivity in Porcine from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HspA6 Antibody - BSA Free

Western Blot: HspA6 Antibody [NBP1-85949]

Western Blot: HspA6 Antibody [NBP1-85949]

Western Blot: HspA6 Antibody [NBP1-85949] - Lane 1: Protein Marker [kDa], Lanes 2 and 3: Porcine endometrium. 40 ug of tissue lysate separated in 4-20% SDS gel and immunoblotted with primary antibody diluted 1:1000. Image from verified customer review.
Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-85949]

Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-85949]

Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-85949] - Staining of human Skin shows moderate nuclear and cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-85949]

Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-85949]

Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-85949] - Staining of human Cerebral cortex shows moderate nuclear positivity in glial cells.
Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-85949]

Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-85949]

Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-85949] - Staining of human Pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-85949]

Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-85949]

Immunohistochemistry-Paraffin: HspA6 Antibody [NBP1-85949] - Staining of human Testis shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.

Applications for HspA6 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. HspA6 Antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-85949 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSPA6/HSP70B

HSP70B is a unique member of the human Hsp70 chaperone involved in cellular processes such as protein trafficking, folding, and prevention of aggregation. This protein is strictly stress-inducible, having little or no basal expression levels in most cells. HSP70B and Hsp72 are closely related and play cooperative roles in cell survival of proteotoxic stress. Recombinant human HSP70B, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Long Name

Heat Shock Protein A6

Alternate Names

HSP70B

Gene Symbol

HSPA6

Additional HSPA6/HSP70B Products

Product Documents for HspA6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for HspA6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HspA6 Antibody - BSA Free

Customer Reviews for HspA6 Antibody - BSA Free (1)

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Customer Images

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  • HspA6 Antibody
    Name: Adrianne Kaiser-Vry
    Application: Western Blot
    Sample Tested: Porcine endometrium
    Species: Porcine
    Verified Customer | Posted 09/11/2018
    Western Blot: HspA6 Antibody [NBP1-85949] Lane 1: Protein Marker [kDa] Lanes 2 and 3: Porcine endometrium ; 40ug of tissue lysate separated in 4-20% SDS gel and immunoblotted with primary antibody diluted @ 1:1000.
    HspA6 Antibody - BSA Free NBP1-85949

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Protocols

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FAQs

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