HTRA1/PRSS11 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-23869
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Canine, Primate
Cited:
Human
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Within the range of amino acids 234-262of human HtrA1 were used as the immunogen, Gen Bank no NP_002766.1. This peptide sequence is in the serine protease domain of the HtrA1 protein.
Reactivity Notes
This antibody is reactive to Chimpanzee
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HTRA1/PRSS11 Antibody - BSA Free
Western Blot: HTRA1/PRSS11 Antibody [NBP2-23869]
Western Blot: HTRA1/PRSS11 Antibody [NBP2-23869] - Analysis of HtrA1 in human A) HeLa cell lysate (1 minute exposure) and B) human 293 cell lysate (15 minute exposure) using NBP2-23869 at 1:500.Simple Western: HTRA1/PRSS11 Antibody [NBP2-23869]
Simple Western: HTRA1/PRSS11 Antibody [NBP2-23869] - Simple Western lane view shows a specific band for htrA1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for HTRA1/PRSS11 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Simple Western
1:5000
Western Blot
1:250-1: 1000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:5000, apparent MW was 67 kDa.
See Simple Western Antibody Database for Simple Western validation: Tested in Hek293 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:5000, apparent MW was 67 kDa.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole sera with 50% Glycerol
Format
BSA Free
Preservative
0.025% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HTRA1/PRSS11
Long Name
High Temperature Requirement Protein-1
Alternate Names
IGFBP5-protease, PRSS11
Gene Symbol
HTRA1
UniProt
Additional HTRA1/PRSS11 Products
Product Documents for HTRA1/PRSS11 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for HTRA1/PRSS11 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for HTRA1/PRSS11 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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