HtrA3 Antibody - BSA Free

Novus Biologicals | Catalog # NB600-1151

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Feline (93%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HTRA3 Primary Antibodies »

Product Specifications

Immunogen

Amino acids 273-287 (IVSTAQREGRELGLR) of human HtrA3 were used as immunogen.

Reactivity Notes

Predicted to react with Chicken and Feline

Localization

Secreted

Specificity

The amino acid sequence is 100% homologous in HtrA3, 80% homologous in HtrA1 and 71% homologous in HtrA2 and HtrA4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HtrA3 Antibody - BSA Free

Western Blot: HtrA3 Antibody [NB600-1151]

Western Blot: HtrA3 Antibody [NB600-1151]

Western Blot: HtrA3 Antibody [NB600-1151] - Western bot analysis of HtrA3 in rat heart lysate using NB600-1151 at 2 ug/ml.
Western Blot: HtrA3 Antibody [NB600-1151]

Western Blot: HtrA3 Antibody [NB600-1151]

Western Blot: HtrA3 Antibody [NB600-1151] - analysis of HtrA1/2/3/4 in heart lysate from A) human, B) mouse and C) rat using this antibody.
HtrA3 Antibody - BSA Free

Western Blot: HtrA3 Antibody - BSA Free [NB600-1151] -

TMAO regulates the expression of DEGs related to patient outcome in TNF-alpha induced inflammatory HCC. (A–D) Western blotting was used to analyze the expression level of Periostin, Snab, Layilin and Htra3 in Hepa1-6 and Huh7 cells treated with 50 ng/ml TNF-alpha combined with 50 μM TMAO or 50 ng/ml TNF-alpha alone. Grayscale values were analyzed; data are mean +/- SD (n = 3 per group); Student’s t-test; ***, p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35676936), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for HtrA3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000. Use reported in scientific literature (Beleford et al)

Western Blot

1:100-1:2000
Application Notes
Western Blot: Detects a band of approximately 43 kDa (predicted molecular weight: 49 kDa).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 0.2% Gelatin

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HTRA3

HtrA3 belongs to the human high-temperature requirement factor A (HtrA) family. HtrA3 has two isoforms (long and short), which are produced by alternative splicing. Similar to HtrA1, HtrA3 binds to various TGF-beta proteins and inhibits the signaling of BMP-4, -2 and TGF-beta 1. HtrA3 does not inhibit signaling originated from a constitutively active BMP receptor, indicating that the inhibition occurs upstream of the cell surface receptor.

Long Name

HtrA serine peptidase 3

Alternate Names

HTRA 3

Entrez Gene IDs

94031 (Human); 78558 (Mouse); 360959 (Rat)

Gene Symbol

HTRA3

UniProt

P83110 (Human)

Additional HTRA3 Products

Product Documents for HtrA3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HtrA3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Serine Proteases and Regulators

Citations for HtrA3 Antibody - BSA Free

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Protocols

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