Human 5-HT2A Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB11250R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB11250R-100UG

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Human 5-HT2A Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects Human HTR2A in Direct ELISA. In flow cytometry, it detects Human HTR2A in HEK293 tranfected cells but it does not detect parental HEK293 or mock irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 1055217
Purification
Protein A or G purified
Immunogen
Synthetic peptide corresponding to the amino acids 1-75 in the N-term extracellular domain of the Serotonin Receptor 5-HT2A (HTR2A).
Accession # P28223
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: 5-HT2A

Human serotonin receptor HTR2A, also known as 5-HT2A or HTR2, is a G-protein coupled receptor (GPCR) for 5-hydroxytryptamine (serotonin). The 5-HT2A receptor is mainly a cell surface receptor with several intracellular locations. Human -5-HT2A is the main excitatory receptor subtype among the GPCRs for serotonin but an inhibitory effect on the visual cortex and the orbitofrontal cortex has also been described. This receptor was first noted for its importance as a target of serotonergic psychedelic drugs such as LSD and psilocybin mushrooms.

Long Name
5-Hydroxytryptamine Receptor 2A
Entrez Gene IDs
3356 (Human); 15558 (Mouse); 29595 (Rat)
Alternate Names
5-HT2 receptor; 5-HT-2; 5HT2A; 5-HT2A; 5-HT-2A; 5-hydroxytryptamine (serotonin) receptor 2A; 5-hydroxytryptamine receptor 2A; HTR2,5-HT2A; HTR2A; serotonin 5-HT-2A receptor; Serotonin receptor 2A

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