Human 5'-Nucleotidase/CD73 PE-conjugated Antibody

  (3 citations)     
Datasheet / CoA / SDS
Product Details
Citations (3)
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  • Species Reactivity
    Human
  • Specificity
    Detects recombinant human 5'-Nucleotidase in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse 5'-Nucleotidase is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 606112
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human 5'‑Nucleotidase/CD73
    Trp27-Ile511
    Accession # P21589
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of 5'‑Nucleotidase/CD73 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human 5'‑Nucleotidase/CD73 PE‑conjugated Monoclonal Antibody (Catalog # FAB5795P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: 5'-Nucleotidase/CD73

5'-Nucleotidase (also [ecto]-5'-nucleotidase/5'-NT, designated CD73) is a variably glycosylated, 69-73 kDa member of the 5'-Nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3+ Treg lymphocytes, FDCs and B cells. 5'-Nucleotidase hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human 5'-Nucleotidase is a 523 amino acid (aa) GPI-linked protein (aa 27-549). It contains a large Zn‑dependent nucleotidase catalytic region (aa 28-532) and a C-terminal substrate binding site (aa 500-506). On the cell surface it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa 405-454, and a second possesses a 12 aa substitution for aa 253-574. Over aa 1-511, human 5'‑Nucleotidase shares 88% aa identity with both mouse and rat 5'-Nucleotidase.

  • Entrez Gene IDs:
    4907 (Human); 23959 (Mouse); 58813 (Rat)
  • Alternate Names:
    5' nucleotidase (CD73); 5'-NT; 5-NT; 5'Nucleotidase; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Targeted introduction and effective expression of hFIX at the AAVS1 locus in mesenchymal stem cells
    Authors: SJ Li, Y Luo, LM Zhang, W Yang, GG Zhang
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors
    Authors: Christopher J Hewitt
    Biotechnol J, 2016;11(4):473-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.
    Authors: Brady K, Dickinson S, Guillot P, Polak J, Blom A, Kafienah W, Hollander A
    Stem Cells Dev, 2014;23(5):541-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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Isotype Controls
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Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 24  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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