Human CD3 epsilon APC-conjugated Antibody FAB100A-100: R&D Systems

Human CD3 epsilon APC-conjugated Antibody

Clone UCHT1 was used by HLDA to establish CD designation.
(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
  • Source
    Monoclonal Mouse IgG1 Clone # UCHT1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human thymocytes (1)
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # FAB4867P) and either (A) Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD3 epsilon
CD3 epsilon is a key signaling molecule that participates in the formation of the T Cell Receptor (TCR). The alpha beta TCR is composed of six distinct subunits. There is an alpha -beta disulfide-linked heterodimer that recognizes antigen, and this is accompanied by four CD3 subunits that transduce an intracellular signal. CD3 epsilon forms a heterodimer with both CD3 gamma and CD3δ, creating two signaling complexes. These two heterodimers are supplemented by a CD3 zeta zeta homodimer that lies in the center of the TCR complex, generating a fully-functional TCR (2). 
  • References:
    1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
    2. Call, M.E. and K.W. Wucherpfennig (2005) Annu. Rev. Immunol. 23: 101
  • Entrez Gene IDs:
    916 (Human); 12503 (Mouse)
  • Alternate Names:
    CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Effective Cytotoxic T Lymphocyte Targeting of Persistent HIV-1 during Antiretroviral Therapy Requires Priming of Naive CD8+ T Cells
    Authors: Kellie N Smith
    MBio, 2016;7(3):.
  2. Comparative Multi-Donor Study of IFNgamma Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays.
    Authors: Hagen J, Zimmerman R, Goetz C, Bonnevier J, Houchins J, Reagan K, Kalyuzhny A
    Cells, 2015;4(1):84-95.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. HIV-1 infection is characterized by profound depletion of CD161+ Th17 cells and gradual decline in regulatory T cells.
    Authors: Prendergast A, Prado JG, Kang YH, Chen F, Riddell LA, Luzzi G, Goulder P, Klenerman P
    AIDS, 2010;24(4):491-502.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Exogenous TERC alone can enhance proliferative potential, telomerase activity and telomere length in lymphocytes from dyskeratosis congenita patients.
    Authors: Kirwan M, Beswick R, Vulliamy T, Nathwani AC, Walne AJ, Casimir C, Dokal I
    Br. J. Haematol., 2009;144(5):771-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis.
    Authors: Garcia G, Humbert M, Capel F, Rimaniol AC, Escourrou P, Emilie D, Godot V
    Allergy, 2007;62(2):170-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 8  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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