Human Adenosine A2aR Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB94971G-100UG
Detection of Adenosine A2aR in HEK293 Human Cell Line Transfected with Human Adenosine A2aR by Flow Cytometry.
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Human Adenosine A2aR Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Adenosine A2aR in HEK293 human cell line transfected with Human Adenosine A2aR and not irrelavant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 599717
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Adenosine A2aR
Met1-Ser412
Accession # P29274
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HEK293 Human Cell Line Transfected with Human Adenosine A2aR

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry View Larger

Detection of Adenosine A2aR in HEK293 Human Cell Line Transfected with Human Adenosine A2aR by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Adenosine A2aR was stained with Mouse Anti-Human Adora A2R Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB94971G, filled histogram) or Mouse IgG2A isotype control antibody (IC003G, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitution default not known
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Adenosine A2aR

ADORA-A2 (Adenosine A2a Receptor) is a widely expressed seven transmembrane G protein-coupled receptor. Activation by adenosine leads to increased intracellular cAMP levels. ADORA-2A mediates many biological functions, including cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. ADORA-A2 has been identified as a target for therapeutic drugs for inflammation, cancer, ischemic reperfusion injury, diabetic nephropathy, infectious diseases and neuronal disorders.

Long Name
Adenosine A2A Receptor
Entrez Gene IDs
135 (Human); 11540 (Mouse); 25369 (Rat)
Alternate Names
A2aR; adenosine A2 receptor; Adenosine A2a R; adenosine A2a receptor; Adenosine A2aR; adenosine receptor A2a; adenosine receptor subtype A2a; ADORA 2; ADORA2; ADORA2A; hA2aR; RDC8

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