Human Adenosine A2aR PE-conjugated Antibody Summary
Met1-Ser412
Accession # P29274
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Adenosine A2a R in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Adenosine A2a R or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adora A2 R PE-conjugated Monoclonal Antibody (Catalog # FAB94971P). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Adenosine A2aR
ADORA-A2 (Adenosine A2a Receptor) is a widely expressed seven transmembrane G protein-coupled receptor. Activation by adenosine leads to increased intracellular cAMP levels. ADORA-2A mediates many biological functions, including cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. ADORA-A2 has been identified as a target for therapeutic drugs for inflammation, cancer, ischemic reperfusion injury, diabetic nephropathy, infectious diseases and neuronal disorders.
Product Datasheets
Citations for Human Adenosine A2aR PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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CRISPR-Cas9-Based Gene Knockout of Immune Checkpoints in Expanded NK Cells
Authors: Mohammadian Gol, T;Kim, M;Sinn, R;Ureña-Bailén, G;Stegmeyer, S;Gratz, PG;Zahedipour, F;Roig-Merino, A;Antony, JS;Mezger, M;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cooperation between chemotherapy and immune checkpoint blockade to enhance anti-tumour T cell immunity in oesophageal adenocarcinoma
Authors: M Davern, NE Donlon, F O' Connell, AD Sheppard, C Hayes, R King, H Temperley, C Butler, A Bhardwaj, J Moore, D Bracken-Cl, C Donohoe, N Ravi, JV Reynolds, SG Maher, MJ Conroy, J Lysaght
Translational Oncology, 2022-03-30;20(0):101406.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
PD-1 blockade enhances chemotherapy toxicity in oesophageal adenocarcinoma
Authors: M Davern, RM O' Brien, J McGrath, NE Donlon, AM Melo, CE Buckley, AD Sheppard, JV Reynolds, N Lynam-Lenn, SG Maher, J Lysaght
Scientific Reports, 2022-02-28;12(1):3259.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Jurkat cells were treated with PMA/Ionomycin over night and A2AR surface expression was measured
