Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 599717
View all Adenosine A2aR Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Adenosine A2a R
Met1-Ser412
Accession # P29274

Specificity

Detects human Adenosine A2a R in HEK293 human cell line transfected with Human Adenosine A2a R and not irrelavant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Adenosine A2aR PE-conjugated Antibody

Detection of Adenosine A2a R antibody in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP antibody by Flow Cytometry.

Detection of Adenosine A2a R in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human Adenosine A2a R or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adora A2 R PE-conjugated Monoclonal Antibody (Catalog # FAB94971P). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Adenosine A2aR PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP

Reviewed Applications

Read 1 review rated 5 using FAB94971P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Adenosine A2aR

ADORA-A2 (Adenosine A2a Receptor) is a widely expressed seven transmembrane G protein-coupled receptor. Activation by adenosine leads to increased intracellular cAMP levels. ADORA-2A mediates many biological functions, including cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. ADORA-A2 has been identified as a target for therapeutic drugs for inflammation, cancer, ischemic reperfusion injury, diabetic nephropathy, infectious diseases and neuronal disorders.

Long Name

Adenosine A2A Receptor

Alternate Names

A2aR, Adenosine A2a R, ADORA2A, RDC8

Entrez Gene IDs

135 (Human); 11540 (Mouse); 25369 (Rat)

Gene Symbol

ADORA2A

UniProt

P29274

Additional Adenosine A2aR Products

Product Documents for Human Adenosine A2aR PE-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Adenosine A2aR PE-conjugated Antibody

For research use only

Citations for Human Adenosine A2aR PE-conjugated Antibody

Customer Reviews for Human Adenosine A2aR PE-conjugated Antibody (1)

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  • Human Adenosine A2aR PE-conjugated Antibody
    Name: Tahmineh Safaie
    Application: Flow Cytometry
    Sample Tested: Jurkat human acute T cell leukemia cell line
    Species: Human
    Verified Customer | Posted 10/09/2020
    Jurkat cells were treated with PMA/Ionomycin over night and A2AR surface expression was measured
    Human Adenosine A2aR PE-conjugated Antibody FAB94971P

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