Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Bioassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 599717
View all Adenosine A2aR Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Adenosine A2a R
Met1-Ser412
Accession # P29274

Specificity

Detects human Adenosine A2a R in HEK293 human cell line transfected with Human Adenosine A2a R and not irrelavant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Adenosine A2aR Antibody

Detection of Adenosine A2a R antibody in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP antibody by Flow Cytometry.

Detection of Adenosine A2a R in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human Adenosine A2a R or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adora A2 R Monoclonal Antibody (Catalog # MAB9497) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Adenosine A2aR Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Adenosine A2aR

ADORA-A2 (Adenosine A2a Receptor) is a widely expressed seven transmembrane G protein-coupled receptor. Activation by adenosine leads to increased intracellular cAMP levels. ADORA-2A mediates many biological functions, including cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. ADORA-A2 has been identified as a target for therapeutic drugs for inflammation, cancer, ischemic reperfusion injury, diabetic nephropathy, infectious diseases and neuronal disorders.

Long Name

Adenosine A2A Receptor

Alternate Names

A2aR, Adenosine A2a R, ADORA2A, RDC8

Entrez Gene IDs

135 (Human); 11540 (Mouse); 25369 (Rat)

Gene Symbol

ADORA2A

UniProt

P29274

Additional Adenosine A2aR Products

Product Documents for Human Adenosine A2aR Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Adenosine A2aR Antibody

For research use only

Citations for Human Adenosine A2aR Antibody

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FAQs for Human Adenosine A2aR Antibody

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  • Q: What is the light chain isotype for Human Adenosine A2aR Antibody (Catalog # MAB9497)?

    A: The light chain isotype of Catalog # MAB9497 is kappa.

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