Human Adenosine A2aR Antibody

Catalog # Availability Size / Price Qty
Detection of Adenosine A2a R in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP by Flow Cytometry.
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Product Details
Citations (1)
Supplemental Products

Human Adenosine A2aR Antibody Summary

Species Reactivity
Detects human Adenosine A2a R in HEK293 human cell line transfected with Human Adenosine A2a R and not irrelavant transfectants in flow cytometry.
Monoclonal Mouse IgG2A Clone # 599717
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human Adenosine A2a R
Accession # P29274
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Adenosine A2a R antibody in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP antibody by Flow Cytometry. View Larger

Detection of Adenosine A2a R in HEK293 Human Cell Line Transfected with Human Adenosine A2a R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human Adenosine A2a R or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Adora A2 R Monoclonal Antibody (Catalog # MAB9497) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adenosine A2aR

ADORA-A2 (Adenosine A2a Receptor) is a widely expressed seven transmembrane G protein-coupled receptor. Activation by adenosine leads to increased intracellular cAMP levels. ADORA-2A mediates many biological functions, including cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. ADORA-A2 has been identified as a target for therapeutic drugs for inflammation, cancer, ischemic reperfusion injury, diabetic nephropathy, infectious diseases and neuronal disorders.

Long Name
Adenosine A2A Receptor
Entrez Gene IDs
135 (Human); 11540 (Mouse); 25369 (Rat)
Alternate Names
A2aR; adenosine A2 receptor; Adenosine A2a R; adenosine A2a receptor; Adenosine A2aR; adenosine receptor A2a; adenosine receptor subtype A2a; ADORA 2; ADORA2; ADORA2A; hA2aR; RDC8

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Citation for Human Adenosine A2aR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Treatment with intravenous immunoglobulin increases the level of small EVs in plasma of pregnant women with recurrent pregnancy loss
    Authors: MM Jørgensen, R Bæk, J Sloth, K Varming, OB Christians, NE Ditlevsen, N Rajaratnam
    J. Reprod. Immunol., 2020;140(0):103128.
    Species: Human
    Sample Types: Plasma
    Applications: Bioassay


  1. What is the light chain isotype for Human Adenosine A2aR Antibody (Catalog # MAB9497)?

    • The light chain isotype of Catalog # MAB9497 is kappa.

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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