Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry.
BG01V human embryonic stem cells were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB1448R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Alkaline Phosphatase/ALPL
The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.
Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
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