Human Alkaline Phosphatase/ALPL Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1448R-100UG
Detection  of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry.
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Human Alkaline Phosphatase/ALPL Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
Source
Monoclonal Mouse IgG1 Clone # B4-78
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry. View Larger

Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB1448R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

References
  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
Long Name
Alkaline Phosphatase Liver
Entrez Gene IDs
249 (Human); 11647 (Mouse)
Alternate Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935

Product Datasheets

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