Human Alkaline Phosphatase/ALPL APC-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry.
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Citations (5)
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Reviews (1)

Human Alkaline Phosphatase/ALPL APC-conjugated Antibody Summary

Species Reactivity
Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
Monoclonal Mouse IgG1 Clone # B4-78
Protein A or G purified from hybridoma culture supernatant
Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

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Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Alkaline Phosphatase/ALPL antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL APC-conjugated Monoclonal Antibody (Catalog # FAB1448A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a Glycosyl Phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
Long Name
Alkaline Phosphatase Liver
Entrez Gene IDs
249 (Human); 11647 (Mouse)
Alternate Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935

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Citations for Human Alkaline Phosphatase/ALPL APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Protein Expression of AEBP1, MCM4, and FABP4 Differentiate Osteogenic, Adipogenic, and Mesenchymal Stromal Stem Cells
    Authors: T Sauer, G Facchinett, M Kohl, JM Kowal, S Rozanova, J Horn, H Schmal, I Kwee, AP Schulz, S Hartwig, M Kassem, JK Habermann, T Gemoll
    International Journal of Molecular Sciences, 2022;23(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Type 2 diabetes affects bone cells precursors and bone turnover
    Authors: F Sassi, I Buondonno, C Luppi, E Spertino, E Stratta, M Di Stefano, M Ravazzoli, G Isaia, M Trento, P Passera, M Porta, GC Isaia, P D'Amelio
    BMC Endocr Disord, 2018;18(1):55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Negative Association Between Sclerostin and INSL3 in Isolated Human Osteocytes and in Klinefelter Syndrome: New Hints for Testis-Bone Crosstalk
    Authors: A Di Nisio, L De Toni, MS Rocca, M Ghezzi, R Selice, G Taglialavo, A Ferlin, C Foresta
    J. Clin. Endocrinol. Metab., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Interleukin-1beta (IL-1?)-induced Notch ligand Jagged1 suppresses mitogenic action of IL-1? on human dystrophic myogenic cells
    Authors: Y Nagata, T Kiyono, K Okamura, YI Goto, M Matsuo, M Ikemoto-Ue, N Hashimoto
    PLoS ONE, 2017;12(12):e0188821.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010;7(12):989-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Human Alkaline Phosphatase/ALPL APC-conjugated Antibody
By Abby Sukarto on 02/13/2020
Application: Flow Sample Tested: Bone Extracts Species: Human