Home / Alkaline Phosphatase/ALPL / Human Alkaline Phosphatase/ALPL APC-conjugated Antibody

Human Alkaline Phosphatase/ALPL APC-conjugated Antibody

Catalog #: FAB1448A
14 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1448A-100
FAB1448A-025

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Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry.
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Citations (14)
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Reviews (1)
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Human Alkaline Phosphatase/ALPL APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
Source
Monoclonal Mouse IgG1 Clone # B4-78
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
BG01V human embryonic stem cells; iPSCs

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Alkaline Phosphatase/ALPL antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL APC-conjugated Monoclonal Antibody (Catalog # FAB1448A, filled histogram) or isotype control antibody (IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of Alkaline Phosphatase/ALPL in iPSCs by Flow Cytometry iPSCs were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL APC-conjugated Monoclonal Antibody (Catalog # FAB1448A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a Glycosyl Phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

References
  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
Long Name
Alkaline Phosphatase Liver
Entrez Gene IDs
249 (Human); 11647 (Mouse)
Alternate Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935

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Citations for Human Alkaline Phosphatase/ALPL APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Dissection of Cellular Communication between Human Primary Osteoblasts and Bone Marrow Mesenchymal Stem Cells in Osteoarthritis at Single-Cell Resolution
    Authors: Ying Liu, Yan Chen, Xiao-Hua Li, Chong Cao, Hui-Xi Zhang, Cui Zhou et al.
    International Journal of Stem Cells
  2. A miR-125/Sirtuin-7 pathway drives the pro-calcific potential of myeloid cells in diabetic vascular disease
    Authors: Saula Vigili de Kreutzenberg, Alessandra Giannella, Giulio Ceolotto, Elisabetta Faggin, Roberta Cappellari, Marta Mazzucato et al.
    Diabetologia
  3. A New Method of Bone Stromal Cell Characterization by Flow Cytometry
    Authors: Chirayu Patel, Lihong Shi, John F. Whitesides, Brittni M. Foster, Roberto J. Fajardo, Ellen E. Quillen et al.
    Current Protocols
  4. Protein Expression of AEBP1, MCM4, and FABP4 Differentiate Osteogenic, Adipogenic, and Mesenchymal Stromal Stem Cells
    Authors: T Sauer, G Facchinett, M Kohl, JM Kowal, S Rozanova, J Horn, H Schmal, I Kwee, AP Schulz, S Hartwig, M Kassem, JK Habermann, T Gemoll
    International Journal of Molecular Sciences, 2022-02-25;23(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Identification of a clinical signature predictive of differentiation fate of human bone marrow stromal cells
    Authors: Justyna Magdalena Kowal, Sören Möller, Dalia Ali, Florence Figeac, Torben Barington, Hagen Schmal et al.
    Stem Cell Research & Therapy
  6. A systematic dissection of human primary osteoblasts in vivo at single-cell resolution
    Authors: Yun Gong, Junxiao Yang, Xiaohua Li, Cui Zhou, Yu Chen, Zun Wang et al.
    Aging (Albany NY)
  7. Single-cell high-content imaging parameters predict functional phenotype of cultured human bone marrow stromal stem cells
    Authors: Justyna M. Kowal, Hagen Schmal, Ulrich Halekoh, Jacob B. Hjelmborg, Moustapha Kassem
    Stem Cells Translational Medicine
  8. Type 2 diabetes affects bone cells precursors and bone turnover
    Authors: F Sassi, I Buondonno, C Luppi, E Spertino, E Stratta, M Di Stefano, M Ravazzoli, G Isaia, M Trento, P Passera, M Porta, GC Isaia, P D'Amelio
    BMC Endocr Disord, 2018-08-08;18(1):55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Negative Association Between Sclerostin and INSL3 in Isolated Human Osteocytes and in Klinefelter Syndrome: New Hints for Testis-Bone Crosstalk
    Authors: A Di Nisio, L De Toni, MS Rocca, M Ghezzi, R Selice, G Taglialavo, A Ferlin, C Foresta
    J. Clin. Endocrinol. Metab., 2018-05-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Interleukin-1beta (IL-1?)-induced Notch ligand Jagged1 suppresses mitogenic action of IL-1? on human dystrophic myogenic cells
    Authors: Y Nagata, T Kiyono, K Okamura, YI Goto, M Matsuo, M Ikemoto-Ue, N Hashimoto
    PLoS ONE, 2017-12-01;12(12):e0188821.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Osteogenic monocytes within the coronary circulation and their association with plaque vulnerability in patients with early atherosclerosis
    Authors: Julia Collin, Mario Gössl, Yoshiki Matsuo, Rebecca R. Cilluffo, Andreas J. Flammer, Darrell Loeffler et al.
    International Journal of Cardiology
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. The C-terminus of tissue factor pathway inhibitor alpha is required for its interaction with factors V and Va.
    J. Thromb. Haemost., 2012-09-01;10(9):1944-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study
    Authors: Michael D. Ball, Ian C. Bonzani, Melissa J. Bovis, Andrew Williams, Molly M. Stevens
    Clinical Orthopaedics & Related Research
  14. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010-11-14;7(12):989-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human Alkaline Phosphatase/ALPL APC-conjugated Antibody
By Abby Sukarto on 02/13/2020
Application: Flow Sample Tested: Bone Extracts Species: Human
Flow Cytometry Human Alkaline Phosphatase/ALPL APC-conjugated Antibody FAB1448A