Human Alkaline Phosphatase/
ALPL Biotinylated Antibody

Catalog # Availability Size / Price Qty
Alkaline Phosphatase/ALPL in Human Osteoblasts.
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Product Details
Citations (3)
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Reviews (1)

Human Alkaline Phosphatase/
ALPL Biotinylated Antibody Summary

Species Reactivity
Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
Monoclonal Mouse IgG1 Clone # B4-78
Protein A or G purified from hybridoma culture supernatant
Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood granulocytes
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human intestine
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Alkaline Phosphatase/ALPL antibody in Human Osteoblasts by Immunocytochemistry (ICC). View Larger

Alkaline Phosphatase/ALPL in Human Osteoblasts. Alkaline Phosphatase/ALPL was detected in immersion fixed differentiated human osteoblasts using Human Alkaline Phosphatase/ALPL Biotinylated Monoclonal Antibody (Catalog # BAM1448) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
Long Name
Alkaline Phosphatase Liver
Entrez Gene IDs
249 (Human); 11647 (Mouse)
Alternate Names
Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935

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Citations for Human Alkaline Phosphatase/
ALPL Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Activation of cancer-related and mitogen-activated protein kinase signaling pathways in human mature osteoblasts isolated from patients with type 2 diabetes
    Authors: T Kuroiwa, M Matsumoto, R Kato, A Nimura, T Yoshii, A Okawa, K Fujita
    Bone Rep, 2019;10(0):100199.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection
  2. Effects of intermittent parathyroid hormone treatment on osteoprogenitor cells in postmenopausal women.
    Authors: Drake MT, Srinivasan B, Modder UI, Ng AC, Undale AH, Roforth MM, Peterson JM, McCready LK, Riggs BL, Khosla S
    Bone, 2011;49(3):349-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering.
    Authors: Pytlik R, Stehlik D, Soukup T, Kalbacova M, Rypacek F, Trc T, Mulinkova K, Michnova P, Kideryova L, Zivny J, Klener P, Vesela R, Trneny M, Klener P
    Biomaterials, 2009;30(20):3415-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Reviews for Human Alkaline Phosphatase/
ALPL Biotinylated Antibody

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Human Alkaline Phosphatase/ALPL Biotinylated Antibody
By Adam Guess on 11/08/2016
Application: Flow Sample Tested: Differentiated osteoblasts Species: Mouse