Human Alkaline Phosphatase/ALPL Biotinylated Antibody

(2 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
  • Source
    Monoclonal Mouse IgG1 Clone # B4-78
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human peripheral blood granulocytes
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human intestine
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Alkaline Phosphatase/ALPL in Human Osteoblasts.

Alkaline Phosphatase/ALPL was detected in immersion fixed differentiated human osteoblasts using Human/Mouse/Rat Alkaline Phosphatase/ALPL Biotinylated Monoclonal Antibody (Catalog # BAM1448) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Alkaline Phosphatase/ALPL
The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.
  • References:
    1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
    2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
    3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
  • Long Name:
    Alkaline Phosphatase Liver
  • Entrez Gene IDs:
    249 (Human); 11647 (Mouse)
  • Alternate Names:
    Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Effects of intermittent parathyroid hormone treatment on osteoprogenitor cells in postmenopausal women.
    Authors: Drake MT, Srinivasan B, Modder UI, Ng AC, Undale AH, Roforth MM, Peterson JM, McCready LK, Riggs BL, Khosla S
    Bone, 2011;49(3):349-55.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering.
    Authors: Pytlik R, Stehlik D, Soukup T, Kalbacova M, Rypacek F, Trc T, Mulinkova K, Michnova P, Kideryova L, Zivny J, Klener P, Vesela R, Trneny M, Klener P
    Biomaterials, 2009;30(20):3415-27.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Biotinylated Antibody

Ctrl IC002B 2
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Staining Reagents
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Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human Alkaline Phosphatase/ALPL Biotinylated Antibody BAM1448
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Very Good
 Flow Mouse Anonymous 11/08/2016
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Flow Cytometry Human Alkaline Phosphatase/ALPL Biotinylated Antibody BAM1448
Flow Cytometry: Human Alkaline Phosphatase/ALPL Biotinylated Antibody [BAM1448]

Summary

ApplicationFlow Cytometry
Sample TestedDifferentiated osteoblasts
SpeciesMouse

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