Human Alkaline Phosphatase/ALPP Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB59051R Datasheet / COA / SDS

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FAB59051R-100UG

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Human Alkaline Phosphatase/ALPP Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Alkaline Phosphatase/ALPP in direct ELISAs. In direct ELISAs, this antibody cross-reacts at 100% with recombinant human (rh) ALPPL2, but no cross-reactivity with rhAlkaline Phosphatase/ALPI is observed.

Source

Monoclonal Mouse IgG1 Clone # 696128

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Alkaline Phosphatase/ALPP
Ile23-Asp506
Accession # P05187

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Alkaline Phosphatase/ALPP

Four distinct genes encode alkaline phosphatases (APs) in humans. The ALPP gene encodes the 67 kDa placental isozyme, also known as PLAP. In comparison, ALPI, ALPL, and ALPPL2 encode intestinal, liver/bone/kidney, and placental-like or germ cell APs, respectively. The placental alkaline phosphatase is a glycosyl phosphatidylinositol (GPI) anchored protein that is expressed on the cell surface as a homodimer. Human APs are ectopically expressed in several cancers, and their serum levels are useful tumor markers. Mature human Alkaline Phosphatase/ALPP shares 74% amino acid sequence homology with the mouse and rat enzymes.

Long Name

Alkaline Phosphatase, Placental Type

Entrez Gene IDs

250 (Human); 11650 (Mouse); 367308 (Rat)

Alternate Names

Alkaline phosphatase Regan isozyme; Alkaline Phosphatase; alkaline phosphatase, placental (Regan isozyme); alkaline phosphatase, placental type; alkaline phosphatase, placental; alkaline phosphomonoesterase; ALP; ALPP; EC 3.1.3.1; FLJ61142; glycerophosphatase; PALP; Placental alkaline phosphatase 1; PLAP; PLAP-1; Regan isozyme; SEAP