Human alpha 1-Acid Glycoprotein Quantikine ELISA Kit

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Human alpha 1-Acid Glycoprotein ELISA Standard Curve
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Product Details
Citations (6)
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Human alpha 1-Acid Glycoprotein Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
0.538 ng/mL
Assay Range
3.1 - 200 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Natural human AGP
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human AGP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human AGP in cell culture supernates, serum, plasma, and urine.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 16.5 45.8 105 16.7 45.2 105
Standard Deviation 0.87 1.96 7.75 1.11 2.96 8.6
CV% 5.3 4.3 7.4 6.7 6.6 8.2


The recovery of AGP spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 85-111
Urine (n=4) 96 86-111


To assess the linearity of the assay, samples containing or spiked with high concentrations of AGP were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human alpha 1-Acid Glycoprotein ELISA Linearity

Scientific Data

Human alpha 1-Acid Glycoprotein ELISA Standard Curve

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: alpha 1-Acid Glycoprotein

Alpha 1-acid Glycoprotein (AGP), also known as Orosomucoid, is a Lipocalin family acute-phase protein that is produced by the liver in response to inflammation and infection. Its serum concentration can increase 2-5 fold, making it one of the predominant proteins in serum. AGP functions as a carrier protein for many basic and neutral lipophilic drugs including steroid hormones. It can inhibit neutrophil migration in sepsis, exert a protective effect in severe bacterial infection, and inhibit platelet aggregation. The glycosylation pattern of alpha 1-Acid Glycoprotein influences its anti-inflammatory properties and is associated with various pathophysiological states.

Entrez Gene IDs:
5004 (Human); 18405 (Mouse); 24614 (Rat)
Alternate Names:
AGP 1; AGP; AGP1; AGP1alpha-1-acid glycoprotein 1; AGPA; AGP-A; alpha 1Acid Glycoprotein; alpha 1-Acid Glycoprotein; OMD 1; ORM; ORM1; orosomucoid 1; Orosomucoid; orosomucoid-1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human alpha 1-Acid Glycoprotein Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Associations of maternal iron deficiency with malaria infection in a cohort of pregnant Papua New Guinean women
    Authors: HW Unger, A Bleicher, M Ome-Kaius, EH Aitken, SJ Rogerson
    Malaria Journal, 2022;21(1):153.
    Species: Human
    Sample Types: Plasma
  2. The relationship between markers of antenatal iron stores and birth outcomes differs by malaria prevention regimen-a prospective cohort study
    Authors: HW Unger, V Laurita Lo, A Bleicher, M Ome-Kaius, S Karl, JA Simpson, A Karahalios, EH Aitken, SJ Rogerson
    Bmc Medicine, 2021;19(1):236.
    Species: Human
    Sample Types: blood
  3. Sulphadoxine-pyrimethamine plus azithromycin may improve birth outcomes through impacts on inflammation and placental angiogenesis independent of malarial infection
    Authors: HW Unger, AP Hansa, C Buffet, W Hasang, A Teo, L Randall, M Ome-Kaius, S Karl, AA Anuan, JG Beeson, I Mueller, SJ Stock, SJ Rogerson
    Sci Rep, 2019;9(1):2260.
    Species: Human
    Sample Types: Plasma
  4. Hypoallergenic and anti-inflammatory feeds in children with complicated severe acute malnutrition: an open randomised controlled 3-arm intervention trial in Malawi
    Authors: RH Bartels, E Chimwezi, V Watson, L Pei, I Potani, B Allubha, K Chidzalo, D Wang, Q Dube, M Mallewa, A Allen, RHJ Bandsma, WP Voskuijl, SJ Allen
    Sci Rep, 2019;9(1):2304.
    Species: Human
    Sample Types: Plasma
  5. Identification of novel biomarkers in seasonal allergic rhinitis by combining proteomic, multivariate and pathway analysis.
    Authors: Wang H, Gottfries J, Barrenas F, Benson M
    PLoS ONE, 2011;6(8):e23563.
    Species: Human
    Sample Types: Nasal Fluid
  6. The effects of fat loss after bariatric surgery on inflammation, serum hepcidin, and iron absorption: a prospective 6-mo iron stable isotope study.
    Authors: Cepeda-Lopez A, Allende-Labastida J, Melse-Boonstra A, Osendarp S, Herter-Aeberli I, Moretti D, Rodriguez-Lastra R, Gonzalez-Salazar F, Villalpando S, Zimmermann M
    Am J Clin Nutr, 0;104(4):1030-1038.
    Species: Human
    Sample Types: Serum


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