Human alpha 1-Acid Glycoprotein Quantikine ELISA Kit Summary
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Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of AGP spiked to levels throughout the range of the assay in various matrices was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=5)||98||85-111|
Human alpha 1-Acid Glycoprotein ELISA Standard Curve
Preparation and Storage
Background: alpha 1-Acid Glycoprotein
Alpha 1-acid Glycoprotein (AGP), also known as Orosomucoid, is a Lipocalin family acute-phase protein that is produced by the liver in response to inflammation and infection. Its serum concentration can increase 2-5 fold, making it one of the predominant proteins in serum. AGP functions as a carrier protein for many basic and neutral lipophilic drugs including steroid hormones. It can inhibit neutrophil migration in sepsis, exert a protective effect in severe bacterial infection, and inhibit platelet aggregation. The glycosylation pattern of alpha 1-Acid Glycoprotein influences its anti-inflammatory properties and is associated with various pathophysiological states.
Assay ProcedureRefer to the product
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human alpha 1-Acid Glycoprotein Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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Associations of maternal iron deficiency with malaria infection in a cohort of pregnant Papua New Guinean women
Authors: HW Unger, A Bleicher, M Ome-Kaius, EH Aitken, SJ Rogerson
Malaria Journal, 2022;21(1):153.
Sample Types: Plasma
The relationship between markers of antenatal iron stores and birth outcomes differs by malaria prevention regimen-a prospective cohort study
Authors: HW Unger, V Laurita Lo, A Bleicher, M Ome-Kaius, S Karl, JA Simpson, A Karahalios, EH Aitken, SJ Rogerson
Bmc Medicine, 2021;19(1):236.
Sample Types: blood
Sulphadoxine-pyrimethamine plus azithromycin may improve birth outcomes through impacts on inflammation and placental angiogenesis independent of malarial infection
Authors: HW Unger, AP Hansa, C Buffet, W Hasang, A Teo, L Randall, M Ome-Kaius, S Karl, AA Anuan, JG Beeson, I Mueller, SJ Stock, SJ Rogerson
Sci Rep, 2019;9(1):2260.
Sample Types: Plasma
Hypoallergenic and anti-inflammatory feeds in children with complicated severe acute malnutrition: an open randomised controlled 3-arm intervention trial in Malawi
Authors: RH Bartels, E Chimwezi, V Watson, L Pei, I Potani, B Allubha, K Chidzalo, D Wang, Q Dube, M Mallewa, A Allen, RHJ Bandsma, WP Voskuijl, SJ Allen
Sci Rep, 2019;9(1):2304.
Sample Types: Plasma
Identification of novel biomarkers in seasonal allergic rhinitis by combining proteomic, multivariate and pathway analysis.
Authors: Wang H, Gottfries J, Barrenas F, Benson M
PLoS ONE, 2011;6(8):e23563.
Sample Types: Nasal Fluid
The effects of fat loss after bariatric surgery on inflammation, serum hepcidin, and iron absorption: a prospective 6-mo iron stable isotope study.
Authors: Cepeda-Lopez A, Allende-Labastida J, Melse-Boonstra A, Osendarp S, Herter-Aeberli I, Moretti D, Rodriguez-Lastra R, Gonzalez-Salazar F, Villalpando S, Zimmermann M
Am J Clin Nutr, 0;104(4):1030-1038.
Sample Types: Serum
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