Detection of Aminopeptidase N/CD13 in Human Blood Granulocytes by Flow Cytometry.
Human peripheral blood granulocytes were stained with Mouse Anti-Human Aminopeptidase N/CD13 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB8284G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Aminopeptidase N/CD13
The human ANPEP gene encodes Aminopeptidase N (APN), which is also known as Microsomal Aminopeptidase, Alanyl Aminopeptidase, Aminopeptidase M, CD13, or Membrane Protein p161 (1-3). The deduced amino acid sequence of human APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). The amino acid sequence of human APN is 78% and 77% identical to that of rat and mouse, respectively. Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (3).
Olsen, J. et al. (1988) FEBS Lett. 238:307.
Look, A.T. et al. (1989) J. Clin. Invest. 83:1299.
Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 289, Academic Press, San Diego.
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