Human APRIL/TNFSF13 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human APRIL/TNFSF13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Fas Ligand, rhTNF-alpha, rhTRAIL, and rhTRANCE is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human APRIL/TNFSF13
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of APRIL/TNFSF13 in Th2-stimulated PBMCs by Flow Cytometry. Th2-stimulated peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human APRIL/TNFSF13 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC884P) and Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # IC108P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: APRIL/TNFSF13
APRIL (A Proliferation-inducing Ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily. APRIL is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the N- or C-terminus. Human APRIL shares 85% aa sequence identity with mouse and rat APRIL. Among TNF superfamily ligands, BAFF shows the greatest sequence homology with APRIL, and the two proteins exhibit overlapping biological activities. APRIL promotes cellular proliferation and provides protection from apoptosis in normal and transformed cells. It is present in elevated amounts in a wide variety of cancers, primarily due to expression by tumor-infiltrating neutrophils, and is known to be expressed by T cells, macrophages, B cells, adipocytes and capillary endothelial cells. Both APRIL and BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, and BAFF additionally functions through BAFF R. A stretch of basic amino acids at the N-terminus of APRIL is required for its interaction with Heparan Sulfate Proteoglycans (HSPGs). Binding to HSPGs is independent of APRIL’s binding to TACI and BCMA. Interaction with HSPGs serves to concentrate APRIL on the surface of cells, thereby favoring TACI- or BCMA-mediated effects. APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders. A bioactive protein known as TWE-PRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. TWE-PRIL is expressed in monocytes and activated T cells, and in contrast to APRIL, is presented on the cell surface.
  • Long Name:
    A Proliferation-inducing Ligand
  • Entrez Gene IDs:
    8741 (Human); 69583 (Mouse)
  • Alternate Names:
    A proliferation-inducing ligand; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG PE-conjugated Antibody

Ctrl IC108P 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human APRIL/TNFSF13 PE-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information