Human APRIL/TNFSF13 Antibody

Catalog # Availability Size / Price Qty
AF884
AF884-SP
Human APRIL/TNFSF13 ELISA Standard Curve.
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Product Details
Citations (3)
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Human APRIL/TNFSF13 Antibody Summary

Species Reactivity
Human
Specificity
Detects human APRIL/TNFSF13 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Fas Ligand, rhTNF-alpha, rhTRAIL, and rhTRANCE is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human APRIL/TNFSF13
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human APRIL/TNFSF13 (Catalog # 884-AP)
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB8844).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human APRIL/TNFSF13 DuoSet ELISA Kit (Catalog # DY884B) for convenient development of a sandwich ELISA.

 
Flow Cytometry
0.25 µg/106 cells
U937 human lymphoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Human APRIL/TNFSF13 ELISA Standard Curve. Recombinant Human APRIL/TNFSF13 protein was serially diluted 2-fold and captured by Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (MAB8844) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Intracellular Staining by Flow Cytometry View Larger

Detection of APRIL/TNFSF13 in U937 Human Cell Line by Flow Cytometry. U937 human lymphoma cell line was stained with Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884, filled histogram) or control antibody staining (AB-108-C, open histogram) followed by anti-Goat IgG PE-conjugated Secondary Antibody (F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APRIL/TNFSF13

APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily (1). APRIL is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule (2, 3). Secreted APRIL consists almost entirely of a single TNF homology domain (2, 3). Little or no transmembrane APRIL is expressed on the cell surface (3). Alternate splicing generates isoforms with short deletions at the N- or C-terminus (4). Human APRIL shares 85% aa sequence identity with mouse and rat APRIL. Among TNF superfamily ligands, BAFF shows the greatest sequence homology with APRIL, and the two proteins exhibit overlapping biological activities. APRIL promotes cellular proliferation and protects from apoptosis in normal and transformed cells (3, 5‑7). It is present in elevated amounts in a wide variety of cancers primarily due to expression by tumor-infiltrating neutrophils (4, 5, 7‑9). Both APRIL and BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, and BAFF additionally functions through BAFF R (6, 10, 11). A stretch of basic amino acids at the N-terminus of APRIL is required for its interaction with heparan sulfate proteoglycans (HSPGs) (12, 13). Binding to HSPGs is independent of APRIL’s binding to TACI and BCMA (12, 13). Interaction with HSPGs serves to concentrate APRIL on the surface of cells, thereby favoring TACI- or BCMA-mediated effects (8, 9, 13). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (14). A bioactive protein known as TWE-PRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (15). TWE-PRIL is expressed in monocytes and activated T cells, and in contrast to APRIL, it is presented on the cell surface (15).

References
  1. Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
  2. Hahne, M. et al. (1998) J. Exp. Med. 188:1185.
  3. Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
  4. Kelly, K. et al. (2000) Cancer Res. 60:1021.
  5. Roth, W. et al. (2001) Cell Death Differ. 8:403.
  6. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  7. He, B. et al. (2004) J. Immunol. 172:3268.
  8. Schwaller, J. et al. (2007) Blood 109:331.
  9. Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
  10. Day, E.S. et al. (2005) Biochemistry 44:1919.
  11. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
  12. Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
  13. Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
  14. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  15. Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.
Long Name
A Proliferation-inducing Ligand
Entrez Gene IDs
8741 (Human); 69583 (Mouse); 287437 (Rat)
Alternate Names
A proliferation-inducing ligand; APRIL; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2

Product Datasheets

Citations for Human APRIL/TNFSF13 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells
    Authors: H Talbot, S Saada, E Barthout, PF Gallet, N Gachard, J Abraham, A Jaccard, D Troutaud, F Lalloué, T Naves, AL Fauchais, MO Jauberteau
    Sci Rep, 2020;10(1):12572.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  2. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012;209(10):1813-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone.
    Authors: Moreaux J, Legouffe E, Jourdan E, Quittet P, Reme T, Lugagne C, Moine P, Rossi JF, Klein B, Tarte K
    Blood, 2004;103(8):3148-57.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

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