Human Axl Quantikine ELISA Kit

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DAXL00
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Human Axl ELISA Standard Curve
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Human Axl Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL), Urine (10 uL), Cerebrospinal Fluid (10 uL)
Sensitivity
4.6 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid)
Specificity
Natural and recombinant human Axl
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human Axl Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Axl in cell culture supernates, cell lysates, cerebrospinal fluid, serum, plasma, saliva, and urine. It contains
NS0-expressed recombinant human Axl and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Axl showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Axl.

Precision

Intra-Assay Precision (Precision within an assay) <div>Three samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) <div>Three samples of known concentration were tested in twenty separate assays to assess&nbsp;inter-assay precision. Assays were performed by at least three technicians.</div>

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 249 565 1115 258 582 1131
Standard Deviation 4.21 10 18.6 11.3 19.4 42.4
CV% 1.7 1.8 1.7 4.4 3.3 3.7

Recovery

The recovery of human Axl spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 89 81-98
Cell Lysis Buffer (n=1) 90 88-93
Saliva (n=4) 94 82-101

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Axl were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Axl ELISA Linearity

Data Examples

Human Axl ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse orthologs of chicken c-Eyk) constitute the TAM receptor tyrosine kinase subfamily. This RTK subfamily is characterized by an extracellular domain that consists of two Ig-like motifs and two fibronectin type III motifs. These receptors bind the vitamin K-dependent protein Growth Arrest Specific Gene 6 (Gas6). Receptor activation leads to cell proliferation, migration, or the prevention of apoptosis. Cellular signaling through this family of RTKs is involved in hematopoiesis, embryonic development, tumorigenesis, and spermatogenesis.

Long Name:
Axl Receptor Tyrosine Kinase
Entrez Gene IDs:
558 (Human); 26362 (Mouse)
Alternate Names:
Ark; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; tyrosine-protein kinase receptor UFO; Ufo; UFOoncogene AXL

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