Human B7-H2 PE-conjugated Antibody

(1 Review)
  • Species Reactivity
  • Specificity
    Detects human B7-H2 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H3, or recombinant mouse B7-H2 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 136726
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human B7-H2
    Accession # O75144
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of B7‑H2 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human B7‑H2 PE‑conjugated Monoclonal Antibody (Catalog # FAB165P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B7-H2

Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a 60 kDa member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20‑25% amino acid identity. Within the extracellular domain, human B7-H2 shares 49% and 54% amino acid sequence identity with human and rat B7-H2, respectively. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 amino acid (aa) protein with a putative 18 aa signal peptide, a 239 aa extracellular domain, an 18 aa transmembrane region, and a 33 aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7‑H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

  • References:
    1. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
    2. Ling, V. et al. (2000) J. Immunol. 164:1653.
    3. Wang, S. et al. (2000) Blood 96:2808.
    4. Brodie, D. et al. (2000) Curr. Biol. 10:333.
    5. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
    6. Swallow, M.M. et al. (1999) Immunity 11:423.
    7. Yoshinaga, S.K. et al. (1999) Nature 402:827.
  • Long Name:
    B7 Homolog 2
  • Entrez Gene IDs:
    23308 (Human); 50723 (Mouse); 102132485 (Cynomolgus Monkey)
  • Alternate Names:
    B7H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 12  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human B7‑H2 PE‑conjugated Antibody FAB165P
 Flow Human Anonymous 10/26/2015
Image Details
Flow Cytometry Human B7‑H2 PE‑conjugated Antibody FAB165P
Flow Cytometry: Human B7‑H2 PE‑conjugated Antibody [FAB165P]


ApplicationFlow Cytometry
Sample TestedPeripheral blood cells

Other Experimental Details

Other Experimental DetailsGated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Specificity: Specific
Sensitivity: Sensitive
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100

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