Detects human B7-H2 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H3, or recombinant mouse B7-H2 is observed.
Monoclonal Mouse IgG2B Clone # 136726
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human B7-H2 Asp19-Ser258 Accession # O75144
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
2.5 µg/106 cells
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Measured by its ability to neutralize B7‑H2-induced proliferation in PHA-activated human T cells. Wang, S. et al. (2000) Blood 96:2808. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera and 20 ng/mL Human CD3 epsilon Monoclonal Antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by B7‑H2 and Neutralization by Human B7‑H2 Antibody. In the presence of Human CD3 epsilon Monoclonal Antibody (20 ng/mL, Catalog # MAB100), Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) stimulates proliferation in PHA-activated human T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human B7‑H2 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7‑H2 Monoclonal Antibody (Catalog # MAB165). The ND50 is typically 1-4 µg/mL.
Detection of B7‑H2 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human B7‑H2 Monoclonal Antibody (Catalog # MAB165, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a 60 kDa member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20‑25% amino acid identity. Within the extracellular domain, human B7-H2 shares 49% and 54% amino acid sequence identity with mouse and rat B7-H2, respectively. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 amino acid (aa) protein with a putative 18 aa signal peptide, a 239 aa extracellular domain, an 18 aa transmembrane region, and a 33 aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Ling, V. et al. (2000) J. Immunol. 164:1653.
Wang, S. et al. (2000) Blood 96:2808.
Brodie, D. et al. (2000) Curr. Biol. 10:333.
Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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