Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Development

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all BACE-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BACE-2
Gly63-Pro466
Accession # Q9Y5Z0.1

Specificity

Detects human BACE‑2 Ectodomain in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) BACE-1, rhCathepsin D, and rhCathepsin E is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human BACE-2 Ectodomain Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample:

Human and Mouse BACE‑2 Ectodomain

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BACE-2

BACE-2 ( beta -site APP cleaving enzyme 2; also ASP-1 and Memapsin-1) is a 73‑75 kDa member of the peptidase A1 family. It is expressed both inside and outside the CNS and is classified as a Golgi aspartyl protease. Its status as a beta -secretase is unclear. If it has physiological activity, it may be associated with glia. Mature BACE-2 is a 456 amino acid (aa) type I transmembrane glycoprotein. It contains a 411 aa extracellular domain (aa 63‑473) that has two catalytic residues (Asp93 and Asp289). Two potential splice forms exist; one shows a deletion of aa’s 329‑378, while a second shows an 18 aa substitution for aa’s 380‑518. Over aa 63‑466, human BACE-2 shares 92% aa sequence identity with mouse BACE‑2.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 2

Alternate Names

BACE2

Entrez Gene IDs

25825 (Human); 56175 (Mouse); 288227 (Rat)

Gene Symbol

BACE2

UniProt

Q9Y5Z0.1

Additional BACE-2 Products

Product Documents for Human BACE-2 Ectodomain Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human BACE-2 Ectodomain Biotinylated Antibody

For research use only

Related Research Areas

Aspartic Proteases and Regulators

Citations for Human BACE-2 Ectodomain Biotinylated Antibody

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