Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a 149 kDa transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulatory mechanisms. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites, two poly-Pro regions (aa 514‑517 and 970‑973), and one poly-Ala segment (aa 900‑903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391‑1426. Over aa 1009‑1328, human Bcl-9 is 96% aa identical to mouse Bcl-9. B cell cancers often have translocations at the 3'UTR region of the Bcl-9 gene.
Discontinued Product
MAB3996 has been discontinued.
View all Bcl-9 products.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 417514
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Product Specifications
Immunogen
E. coli-derived recombinant human Bcl-9
Met1009-Gly1328
Accession # O00512
Met1009-Gly1328
Accession # O00512
Specificity
Detects endogenous human Bcl-9 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Bcl‑9 Antibody
Detection of Human Bcl‑9 by Western Blot.
Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human Bcl-9 Monoclonal Antibody (Catalog # MAB3996) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bcl-9 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Bcl‑9 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HCT-116 human colorectal carcinoma cell line and K562 human chronic myelogenous leukemia cell line
Sample: HCT-116 human colorectal carcinoma cell line and K562 human chronic myelogenous leukemia cell line
Reviewed Applications
Read 2 reviews rated 4 using MAB3996 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Bcl-9
Long Name
B Cell Lymphoma/Leukemia 9, Nuclear Co-factor of beta-Catenin Signaling
Alternate Names
Bcl9, BCL9L, LGS
Gene Symbol
BCL9
UniProt
Additional Bcl-9 Products
Product Documents for Human Bcl‑9 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Bcl‑9 Antibody
For research use only
Related Research Areas
Customer Reviews for Human Bcl‑9 Antibody (2)
4 out of 5
2 Customer Ratings
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 12/06/2019Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
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Application: ELISASample Tested: PlasmaSpecies: HumanVerified Customer | Posted 11/10/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
