Detection of Human Bcl‑9 by Western Blot.
Western blot shows lysates of HCT‑116 human colorectal carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human Bcl‑9 Monoclonal Antibody (Catalog # MAB3996) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bcl‑9 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a 149 kDa transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulatory mechanisms. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites, two poly-Pro regions (aa 514‑517 and 970‑973), and one poly-Ala segment (aa 900‑903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391‑1426. Over aa 1009‑1328, human Bcl-9 is 96% aa identical to mouse Bcl-9. B cell cancers often have translocations at the 3'UTR region of the Bcl-9 gene.
B Cell Lymphoma/Leukemia 9, Nuclear Co-factor of beta-Catenin Signaling
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