Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer. BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
Bioassay, ELISA Development, ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 37129
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human BDNF
Arg128-Arg247
Accession # P23560
Arg128-Arg247
Accession # P23560
Specificity
Detects human BDNF in ELISAs. In sandwich ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) GDNF, rh beta -NGF, rhNT-3, or rhNT-4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human BDNF Antibody
Application
Recommended Usage
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL
Reviewed Applications
Read 1 review rated 5 using MAB848 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: BDNF
References
- Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
- Snider, W.D. (1994) Cell 77:627.
- Barbacid, M. (1994) J. Neurobiol. 25:1386.
Long Name
Brain-derived Neurotrophic Factor
Alternate Names
Abrineurin, ANON2, BULN2, Neurotrophin
Gene Symbol
BDNF
UniProt
Additional BDNF Products
Product Documents for Human BDNF Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human BDNF Antibody
For research use only
Related Research Areas
Citations for Human BDNF Antibody
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1 Customer Rating
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/02/2017The MAB648 antibody was used as the capture antibody along with MAB848 as the detection antibody for a sandwich ELISA assay. The calibrator used was recombinant Human BDNF (248-BD). Healthy human serum and plasma samples were all quantifiable at a 1:2 dilution.
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