Human BDNF Antibody Summary
Accession # P23560
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB848R).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human/Mouse BDNF DuoSet ELISA Kit (Catalog # DY248) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human BDNF ELISA Standard Curve. Recombinant Human BDNF protein was serially diluted 2-fold and captured by Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB848R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB648) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.
BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.
- Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
- Snider, W.D. (1994) Cell 77:627.
- Barbacid, M. (1994) J. Neurobiol. 25:1386.
Citations for Human BDNF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells
Authors: H Talbot, S Saada, E Barthout, PF Gallet, N Gachard, J Abraham, A Jaccard, D Troutaud, F Lalloué, T Naves, AL Fauchais, MO Jauberteau
Sci Rep, 2020;10(1):12572.
Sample Types: Recombinant Protein
Applications: Western Blot
Neuroendocrine cells of nasal mucosa are a cellular source of brain-derived neurotrophic factor.
Authors: Jornot L, Lacroix JS, Rochat T
Eur. Respir. J., 2008;32(3):769-74.
Sample Types: Whole Cells
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The MAB648 antibody was used as the capture antibody along with MAB848 as the detection antibody for a sandwich ELISA assay. The calibrator used was recombinant Human BDNF (248-BD). Healthy human serum and plasma samples were all quantifiable at a 1:2 dilution.