Human BDNF Antibody

Catalog # Availability Size / Price Qty
MAB648-SP
MAB648-100
MAB648-500
Human BDNF ELISA Standard Curve.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human BDNF Antibody Summary

Species Reactivity
Human
Specificity
Detects human BDNF in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) NT‑3 or rhNT‑4.
Source
Monoclonal Mouse IgG2A Clone # 37141
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human BDNF
Arg128-Arg247
Accession # P23560
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human BDNF (Catalog # 248-BD). Please note: Human BDNF (clone 35928) (Catalog # MAB248) is preferred for this application.
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB848R).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human/Mouse BDNF DuoSet ELISA Kit (Catalog # DY248) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human BDNF ELISA Standard Curve. Recombinant Human BDNF protein was serially diluted 2-fold and captured by Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB848R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB648) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BDNF

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

References
  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.
Long Name
Brain-derived Neurotrophic Factor
Entrez Gene IDs
627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin

Product Datasheets

Citations for Human BDNF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells
    Authors: H Talbot, S Saada, E Barthout, PF Gallet, N Gachard, J Abraham, A Jaccard, D Troutaud, F Lalloué, T Naves, AL Fauchais, MO Jauberteau
    Sci Rep, 2020;10(1):12572.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  2. Neuroendocrine cells of nasal mucosa are a cellular source of brain-derived neurotrophic factor.
    Authors: Jornot L, Lacroix JS, Rochat T
    Eur. Respir. J., 2008;32(3):769-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human BDNF Antibody

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Human BDNF Antibody
By Anonymous on 11/02/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

The MAB648 antibody was used as the capture antibody along with MAB848 as the detection antibody for a sandwich ELISA assay. The calibrator used was recombinant Human BDNF (248-BD). Healthy human serum and plasma samples were all quantifiable at a 1:2 dilution.