Human BDNF Antibody

Catalog # Availability Size / Price Qty
AF248
AF248-SP
BDNF in Rat Dorsal Root Ganglia.
3 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews

Human BDNF Antibody Summary

Species Reactivity
Human
Specificity
Detects human BDNF in direct ELISAs.
Source
Polyclonal Chicken IgY
Purification
Antigen Affinity-purified from egg yolks
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human BDNF
His129-Arg247
Accession # P23560
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
NTera‑2 human testicular embryonic carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry BDNF in Rat Dorsal Root Ganglia. View Larger

BDNF in Rat Dorsal Root Ganglia. BDNF was detected in perfusion fixed frozen sections of rat dorsal root ganglia using 1 µg/mL Chicken Anti-Human BDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF248) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry BDNF in Rat Dorsal Root Ganglia. View Larger

BDNF in Rat Dorsal Root Ganglia. BDNF was detected in perfusion fixed frozen sections of rat dorsal root ganglia using 1 µg/mL Chicken Anti-Human BDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF248) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry BDNF in Human Spinal Cord. View Larger

BDNF in Human Spinal Cord. BDNF was detected in immersion fixed paraffin-embedded sections of human spinal cord using Chicken Anti-Human BDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF248) at 15 µg/mL overnight at 4 °C. Tissue was stained using HRP-DAB detection (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BDNF

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye, and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung, and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

References
  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.
Long Name
Brain-derived Neurotrophic Factor
Entrez Gene IDs
627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; neurotrophin

Product Datasheets

Citations for Human BDNF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Acid fibroblast growth factor and peripheral nerve grafts regulate Th2 cytokine expression, macrophage activation, polyamine synthesis, and neurotrophin expression in transected rat spinal cords.
    Authors: Kuo HS, Tsai MJ, Huang MC
    J. Neurosci., 2011;31(11):4137-47.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Brain-derived neurotrophic factor mediates non-cell-autonomous regulation of sensory neuron position and identity.
    Authors: Wright MA, Ribera AB,
    J. Neurosci., 2010;30(43):14513-21.
    Species: Zebrafish
    Sample Types: In Vivo
    Applications: In vivo
  3. Interaction between neuropeptide Y (NPY) and brain-derived neurotrophic factor in NPY-mediated neuroprotection against excitotoxicity: a role for microglia.
    Authors: Xapelli S, Bernardino L, Ferreira R, Grade S, Silva AP, Salgado JR, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Oliveira CR, Zimmer J, Malva JO
    Eur. J. Neurosci., 2008;27(8):2089-102.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. PACAP decides neuronal laminar fate via PKA signaling in the developing cerebral cortex.
    Authors: Ohtsuka M, Fukumitsu H, Furukawa S
    Biochem. Biophys. Res. Commun., 2008;369(4):1144-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  5. BDNF stabilizes synapses and maintains the structural complexity of optic axons in vivo.
    Authors: Hu B, Nikolakopoulou AM, Cohen-Cory S
    Development, 2005;132(19):4285-98.
    Species: Xenopus
    Sample Types: In Vivo
    Applications: In vivo

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