Human BDNF Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human BDNF in direct ELISAs.
  • Source
    Polyclonal Chicken IgY
  • Purification
    Antigen Affinity-purified from egg yolks
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human BDNF
    His129-Arg247
    Accession # P23560
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    NTera‑2 human testicular embryonic carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
BDNF in Rat Dorsal Root Ganglia. BDNF was detected in perfusion fixed frozen sections of rat dorsal root ganglia using 1 µg/mL Chicken Anti-Human BDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF248) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
BDNF in Rat Dorsal Root Ganglia. BDNF was detected in perfusion fixed frozen sections of rat dorsal root ganglia using 1 µg/mL Chicken Anti-Human BDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF248) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
BDNF in Human Spinal Cord. BDNF was detected in immersion fixed paraffin-embedded sections of human spinal cord using Chicken Anti-Human BDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF248) at 15 µg/mL overnight at 4 °C. Tissue was stained using HRP-DAB detection (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BDNF

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer.

BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye, and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung, and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor.

  • References:
    1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
    2. Snider, W.D. (1994) Cell 77:627.
    3. Barbacid, M. (1994) J. Neurobiol. 25:1386.
  • Long Name:
    Brain-derived Neurotrophic Factor
  • Entrez Gene IDs:
    627 (Human); 12064 (Mouse); 24225 (Rat)
  • Alternate Names:
    Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; neurotrophin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Acid fibroblast growth factor and peripheral nerve grafts regulate Th2 cytokine expression, macrophage activation, polyamine synthesis, and neurotrophin expression in transected rat spinal cords.
    Authors: Kuo HS, Tsai MJ, Huang MC
    J. Neurosci., 2011;31(11):4137-47.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  2. Brain-derived neurotrophic factor mediates non-cell-autonomous regulation of sensory neuron position and identity.
    Authors: Wright MA, Ribera AB,
    J. Neurosci., 2010;30(43):14513-21.
    Species: Zebrafish
    Sample Type: In Vivo
    Application: In vivo
  3. PACAP decides neuronal laminar fate via PKA signaling in the developing cerebral cortex.
    Authors: Ohtsuka M, Fukumitsu H, Furukawa S
    Biochem. Biophys. Res. Commun., 2008;369(4):1144-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  4. Interaction between neuropeptide Y (NPY) and brain-derived neurotrophic factor in NPY-mediated neuroprotection against excitotoxicity: a role for microglia.
    Authors: Xapelli S, Bernardino L, Ferreira R, Grade S, Silva AP, Salgado JR, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Oliveira CR, Zimmer J, Malva JO
    Eur. J. Neurosci., 2008;27(8):2089-102.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  5. BDNF stabilizes synapses and maintains the structural complexity of optic axons in vivo.
    Authors: Hu B, Nikolakopoulou AM, Cohen-Cory S
    Development, 2005;132(19):4285-98.
    Species: Xenopus
    Sample Type: In Vivo
    Application: In vivo
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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Isotype Controls
Description Application Cat# Citations Images  

Normal Chicken IgY Control

Ctrl AB-101-C 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat Anti-Chicken IgY NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL016 1
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Goat Anti-Chicken IgY NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL017 1
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Goat Anti-Chicken IgY Antibody

WB AF010 1
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Goat Anti-Chicken IgY NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL018
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Goat Anti-Chicken IgY Biotinylated Antibody

WB BAF010
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