Human beta -Catenin Alexa Fluor® 488-conjugated Antibody Summary
Accession # P35222
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of beta ‑Catenin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human beta ‑Catenin Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1329G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
beta -Catenin is a 94-96 kDa member of the beta -Catenin family, armadillo-repeat superfamily of intracellular molecules. Human beta -Catenin is 781 amino acids (aa) in length and contains three basic regions; there is a "flexible" N-terminus (aa 1-110) that undergoes phosphorylation, O-linked glycosylation, acetylation and ubiquitination, a central region (aa 14-670) that contains 12 armadillo-like repeats and engages in protein-protein interaction, and a "flexible" C-terminus that also undergoes phosphorylation. beta -Catenin is widely, if not ubiquitously expressed, being found in endothelial cells, NKT cells, cortical neurons, neural stem cells, osteoblasts, fibroblasts, skeletal muscle cells, and Tregs. beta -Catenin is perhaps best known as a facilitator for Wnt signaling. In this context, and in the absence of Wnt signaling, non-nuclear or E-Cadherin-associated beta -Catenin is continually synthesized and degraded via a phosphorylation-dependent program. Upon Wnt engagement of its LPR:Fzd receptor complex, phosphorylation stops, allowing for an increase in it half-life and translocation into the nucleus. Here, it binds to TCF, generating a gene-activating transcriptional complex. Notably, Wnt signaling has been described as being beta -Catenin and Ca++/non-beta -Catenin based. It now appears they work in tandem, with Ca++ mobilization facilitating the translocation of beta -Catenin across the nuclear membrane. Human and mouse full-length beta -Catenin shows 99% aa sequence identity.
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